LOCUS       sequence-89249-e        2601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2601)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1126..1135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1132..2562
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 cgttcagatc cgctagcgct accggactca gatctcgagg ccaccatggc tgagccccgc
       61 caggagttcg aagtgatgga agatcacgct gggacgtacg ggttggggga caggaaagat
      121 caggggggct acaccatgca ccaagaccaa gagggtgaca cggacgctgg cctgaaagct
      181 gaagaagcag gcattggaga cacccccagc ctggaagacg aagctgctgg tcacgtgacc
      241 caagctcgca tggtcagtaa aagcaaagac gggactggaa gcgatgacaa aaaagccaag
      301 ggggctgatg gtaaaacgaa gatcgccaca ccgcggggag cagcccctcc aggccagaag
      361 ggccaggcca acgccaccag gattccagca aaaaccccgc ccgctccaaa gacaccaccc
      421 agctctggtg aacctccaaa atcaggggat cgcagcggct acagcagccc cggctcccca
      481 ggcactcccg gcagccgctc ccgcaccccg tcccttccaa ccccacccac ccgggagccc
      541 aagaaggtgg cagtggtccg tactccaccc aagtcgccgt cttccgccaa gagccgcctg
      601 cagacagccc ccgtgcccat gccagacctg aagaatgtca agtccaagat cggctccact
      661 gagaacctga agcaccagcc gggaggcggg aaggtgcaaa tagtctacaa accagttgac
      721 ctgagcaagg tgacctccaa gtgtggctca ttaggcaaca tccatcataa accaggaggt
      781 ggccaggtgg aagtaaaatc tgagaagctt gacttcaagg acagagtcca gtcgaagatt
      841 gggtccctgg acaatatcac ccacgtccct ggcggaggaa ataaaaagat tgaaacccac
      901 aagctgacct tccgcgagaa cgccaaagcc aagacagacc acggggcgga gatcgtgtac
      961 aagtcgccag tggtgtctgg ggacacgtct ccacggcatc tcagcaatgt ctcctccacc
     1021 ggcagcatcg acatggtaga ctcgccccag ctcgccacgc tagctgacga ggtgtctgcc
     1081 tccctggcca agcagggttt gagctccgga ggggatccac cggtcgccac catggtgagc
     1141 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     1201 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1261 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     1321 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     1381 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1441 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     1501 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     1561 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     1621 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     1681 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     1741 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     1801 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc
     1861 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     1921 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1981 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     2041 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     2101 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2161 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     2221 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     2281 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     2341 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     2401 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     2461 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     2521 caccacctgt tcctgtacgg catggacgag ctgtacaagt aagcggccgc gactctagat
     2581 cataatcagc cataccacat t
//
