LOCUS       sequence-89251-e        1591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: tdTomato-Mito-7
FEATURES             Location/Qualifiers
     source          1..1591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..95
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      123..132
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..1559
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gtgacgtcag atccgctagc atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag
       61 gctcggcccg gcggctccca gtgccgcgcg ccaagatcca ttcgttgggg gatccaccgg
      121 tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
      181 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      241 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
      301 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
      361 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      421 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
      481 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
      541 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
      601 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
      661 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
      721 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
      781 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
      841 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
      901 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
      961 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     1021 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     1081 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     1141 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     1201 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     1261 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     1321 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     1381 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     1441 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     1501 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaag
     1561 cggccgcgac ttctagatca taatcagcca t
//
