LOCUS       sequence-89253-e        7452 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7452)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MyosinIIA-N-14
FEATURES             Location/Qualifiers
     source          1..7452
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5984..5993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5990..7420
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 taccggactc agatctcgag gccacccaga tccgctagcg ctaccggact cagatctcga
       61 ggccaccatg gctcagcagg ctgcagacaa gtacctctat gtggataaaa acttcatcaa
      121 taacccgctg gcccaagctg actgggctgc caagaagttg gtatgggtgc cttccagcaa
      181 gaatggcttt gaaccagcca gcctcaagga ggaggtggga gaagaggcca ttgtagagct
      241 ggtagagaat gggaagaagg tgaaggtgaa caaggacgac atccagaaga tgaacccacc
      301 caagttctcc aaggtggagg acatggcaga gctcacgtgc ctcaacgaag cttcggtgct
      361 gcacaacctc aaggagcgat actactcagg gcttatctac acctattcag gcctgttctg
      421 tgtggtcatc aacccttata agaacctgcc catctactca gaggagatcg tggagatgta
      481 caagggcaag aagaggcacg agatgccacc ccacatctac gccatcacag atactgccta
      541 ccggagcatg atgcaggacc gggaagatca gtccatcctg tgcacggggg agtctggagc
      601 agggaagaca gagaacacca agaaagtcat ccagtacctg gcacatgtgg cctcctcaca
      661 caagagcaag aaggaccagg gggagttgga gcggcagctg ctacaggcca accctatcct
      721 agaggccttt ggaaacgcca agacggtgaa gaatgacaac tcctctcgat tcggtaaatt
      781 cattcgtatc aactttgatg tcaatggcta cattgttggt gccaacattg agacttatct
      841 tctggagaaa tctcgtgcta tccgccaagc caaagaggag cggaccttcc acatcttcta
      901 ctacctgctg tctggggccg gagaacacct gaagactgat ctcctgttgg agccatacaa
      961 caaataccgc ttcctgtcca acgggcacgt caccatccct gggcagcagg acaaggacat
     1021 gttccaggag acaatggagg ccatgagaat tatgggtatc ccagaggatg agcagatggg
     1081 cttgctgcgg gtcatctctg gggtccttca gcttggcaac attgccttca agaaggagcg
     1141 gaacactgac caggcgtcca tgccggacaa cacagctgct caaaaggtgt cccacctcct
     1201 ggggatcaat gtgaccgact tcaccagagg catcctcacc ccacgcatca aggtgggcag
     1261 agactatgtg cagaaggcgc agactaaaga gcaggctgac tttgccattg aggccttggc
     1321 caaggctacc tatgagcgga tgttccgctg gctggtgctt cgcatcaaca aagctctgga
     1381 caagaccaag aggcagggcg cctcatttat cgggatactg gacatcgctg gctttgaaat
     1441 ctttgatctg aactccttcg agcagctgtg catcaactac accaacgaga agctgcagca
     1501 gctgttcaac cacaccatgt tcatcctgga gcaggaggag taccagcgag agggcatcga
     1561 gtggaacttc atcgacttcg gcctggacct gcagccctgc atcgacctca ttgagaagcc
     1621 ggcgggtccc ccaggcatcc tggccctgct agatgaggag tgctggtttc ctaaggccac
     1681 tgacaagagc ttcgtggaga aggtggtgca ggagcagggc acccacccca agttccagaa
     1741 gcccaagcaa ctgaaggaca aggctgattt ctgcattatc cactatgccg gcaaggtgga
     1801 ctataaagct gacgagtggc tgatgaagaa catggacccc ttgaacgaca acatcgccac
     1861 gctgcttcac cagtcctcag acaagtttgt ctctgagctg tggaaggatg tggatcggat
     1921 cattggcttg gaccaagtgg ctggaatgtc cgagacagca ctacctggtg ccttcaagac
     1981 ccggaagggc atgttccgta ctgtcggaca gctgtacaag gagcagctgg ccaagctcat
     2041 ggccacgttg aggaatacca accccaactt cgtgcgctgc atcattccca accatgagaa
     2101 gaaggccggc aaactggacc cgcacttggt gctggaccag ctgcgctgca atggcgtcct
     2161 tgagggcatc cggatctgcc gccagggctt tcccaacagg gtggtcttcc aggagttccg
     2221 gcagaggtat gagatcctca cccccaactc catcccgaag ggcttcatgg atggcaagca
     2281 agcgtgtgtg ctcatgatca aagccttgga gcttgacagc aacctgtacc gcatcggcca
     2341 gagcaaagtg ttcttccggg caggagtgct cgcccacctg gaggaagagc gggacctgaa
     2401 gatcaccgat gtcatcattg gcttccaggc ctgctgcagg ggctacctgg ccaggaaggc
     2461 ctttgccaag aggcagcaac agctgaccgc catgaaggtc ctacagagga actgtgctgc
     2521 gtacctcagg ctgcgcaact ggcagtggtg gaggctcttc accaaggtca agcccctgtt
     2581 gaactcaata agacatgagg atgagctgtt agccaaggag gcggaactga caaaggttcg
     2641 agagaaacat ctggctgcag agaacaggct gacagagatg gagacgatgc agtctcagct
     2701 catggcagag aagctgcagc ttcaggagca gctgcaggcg gagacagagc tgtgtgccga
     2761 ggctgaggag ctccgggccc gtctgacagc gaagaagcag gagctggagg agatatgcca
     2821 tgacctggag gccagggtgg aggaggagga ggagcgctgc cagtacctgc aggccgagaa
     2881 gaagaagatg cagcagaaca tccaggaact tgaggagcag ttggaggagg aggagagcgc
     2941 ccggcagaag ctgcagcttg agaaggtgac caccgaggcc aagctgaaga aactggagga
     3001 ggaccagatc atcatggagg accagaactg caaactggcc aaggagaaga aactgctgga
     3061 agacagagta gctgagttca ctaccaacct catggaagag gaggagaagt ccaagagcct
     3121 ggccaagctc aagaacaagc acgaggcaat gatcaccgac ctggaagagc gcctccgtag
     3181 ggaggagaag cagaggcagg agttggagaa gacccgtcgc aagctggagg gagactccac
     3241 agacctcagt gaccagattg ctgagctcca ggcgcagata gcagagctca agatgcagct
     3301 ggccaagaag gaggaggagt tgcaggctgc cttggccaga gtggaagaag aagctgctca
     3361 gaagaatatg gccctgaaga agatccgaga actggaaact cagatatctg agctccagga
     3421 ggacctggag tcggagcgag cctccaggaa taaagccgag aagcagaaac gggatctggg
     3481 agaggagctg gaggcgctga agacagagct ggaggacacg ctggactcca cggctgccca
     3541 gcaggagctg aggtcgaagc gtgagcagga ggtgagcatc ctgaagaaga ctctggagga
     3601 cgaggccaag acccatgagg cccagatcca ggagatgagg cagaagcact cacaggctgt
     3661 ggaggagctg gcagatcagt tggagcagac gaagcgggta aaagctaccc ttgagaaggc
     3721 gaagcagacc ctggagaatg agcggggaga gctggccaat gaggtgaagg ccctgctgca
     3781 aggcaagggc gactcagagc acaagcgcaa gaaggtggag gcgcagctgc aagaactgca
     3841 ggtcaagttc agcgagggag agcgcgtgcg aaccgaactg gccgacaagg tcaccaagct
     3901 gcaggttgaa ctggacagtg tgaccggtct ccttagccag tctgacagca agtccagcaa
     3961 gcttacgaag gacttctctg cgctggagtc ccagcttcag gacacacagg agttgctcca
     4021 ggaggagaac cggcagaagc tgagcctgag caccaagctc aagcagatgg aggatgagaa
     4081 aaactccttc agggagcagc tggaggagga ggaggaggcc aagcgcaact tggagaagca
     4141 gatcgccacg ctccatgccc aggtgaccga catgaagaag aagatggagg acggtgtagg
     4201 gtgcctggag actgcagagg aggcgaagcg gaggcttcag aaggacttgg aaggcctgag
     4261 ccagcggctt gaggagaagg tggctgccta cgataagctg gagaagacca agacacggct
     4321 gcagcaggag ctggacgacc tgctggttga cctggaccac cagcggcaga gcgtctccaa
     4381 cctggaaaag aagcagaaga agttcgacca gctcctagcc gaggagaaga ccatctcggc
     4441 caagtatgca gaggagcgtg accgagctga ggctgaggcc cgtgagaagg agacaaaggc
     4501 gctatcactg gcccgggcgc ttgaggaggc catggagcag aaggcagagc tggagcggct
     4561 caacaagcag ttccgcacgg agatggagga cctcatgagc tccaaggatg acgtgggcaa
     4621 gagtgtccac gagctggaga agtccaagcg ggccttggag cagcaggtgg aggagatgaa
     4681 gacccagctg gaggagctgg aggatgagct gcaggccacg gaggatgcca agctccgcct
     4741 ggaggtgaac ctgcaggcca tgaaggccca gtttgagcgg gatctgcagg gccgggatga
     4801 acagagcgag gagaagaaga agcagctggt cagacaggtg cgggagatgg aggcggagct
     4861 ggaggatgag aggaagcagc gctccatggc catggccgca cgcaagaaac tggagatgga
     4921 tctgaaggac ctggaggcac acattgacac agccaataag aaccgggaag aggccatcaa
     4981 acagctgcgg aagcttcagg cccagatgaa ggactgcatg cgggagctgg acgacacgcg
     5041 cgcctcccgg gaggagatcc tggcgcaggc caaggagaat gagaagaagc tgaagagcat
     5101 ggaggccgag atgattcagc tgcaggagga actggcagct gctgagcgtg ctaagcgtca
     5161 ggcccaacag gaacgggacg agctggctga tgagatcgcc aacagcagtg gcaaaggggc
     5221 cctagcatta gaggagaagc ggcgactgga ggcccgcatt gccctgctgg aggaggagct
     5281 ggaggaggaa cagggcaaca cggagctgat caacgatcgg ctgaagaagg ccaacctgca
     5341 gatcgaccaa ataaacaccg acctgaacct ggaacgcagc cacgcacaga agaatgagaa
     5401 tgcgcgacag cagctggaac gccagaacaa ggagctcaag gccaagctgc aggaaatgga
     5461 gagtgctgtc aagtccaaat acaaggcctc catcgcggcc ttggaggcca aaattgcaca
     5521 gctggaggaa cagctggaca acgagaccaa ggagcgccag gcagcctcca agcaggtgcg
     5581 ccggacggag aagaagctga aggacgtgct gctgcaggtg gaggacgagc ggaggaacgc
     5641 ggaacagttc aaggaccagg ctgacaaggc gtccacccgc ctgaagcagc ttaaacggca
     5701 gctagaggag gctgaagagg aggcccagcg ggccaatgcc tcacgccgga agctgcagcg
     5761 tgagctggaa gatgccacag agaccgctga tgctatgaac cgcgaggtca gctccctgaa
     5821 gaacaaactg aggcgtgggg acctgccatt tgtcgtgact cgccgaattg ttcggaaagg
     5881 cactggcgac tgctcagacg aggaggtcga cggtaaagca gatggggccg atgccaaggc
     5941 agctgaaggc ggaagcggcg ggagcgcagg ggatccaccg gtcgccacca tggtgagcaa
     6001 gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     6061 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     6121 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     6181 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     6241 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     6301 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     6361 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     6421 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     6481 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     6541 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     6601 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     6661 gttcctgggg catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga
     6721 ggacaacaac atggccgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc
     6781 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     6841 ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct
     6901 gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga
     6961 ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     7021 cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa
     7081 ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat
     7141 gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat
     7201 ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta
     7261 catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat
     7321 cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca
     7381 ccacctgttc ctgtacggca tggacgagct gtacaagtaa gcggccgcga ctctagatca
     7441 taatcagcca ta
//
