LOCUS       sequence-89254-e        7487 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7487)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MyosinIIB-C-18
FEATURES             Location/Qualifiers
     source          1..7487
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..1462
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggtcat
       61 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
      721 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
      781 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
      841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      901 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
      961 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     1021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     1081 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     1141 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1201 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     1261 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     1321 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     1381 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     1441 cggcatggac gagctgtaca agtccggagg tagcgcatcc agtgggggtg cgtcaggcag
     1501 tggcagcgga tctggcgcgc agagaactgg actcgaggat ccagagaggt atctctttgt
     1561 ggacagggct gtcatctaca accctgccac tcaagctgat tggacagcta aaaagctagt
     1621 gtggattcca tcagaacgcc atggttttga ggcagctagt atcaaagaag aacggggaga
     1681 tgaagttatg gtggagttgg cagagaatgg aaagaaagca atggtcaaca aagatgatat
     1741 tcagaagatg aacccaccta agttttccaa ggtggaggat atggcagaat tgacatgctt
     1801 gaatgaagct tccgttttac ataatctgaa ggatcgctac tattcaggac taatctatac
     1861 ttattctgga ctcttctgtg tagttataaa cccttacaag aatcttccaa tttactctga
     1921 gaatattatt gaaatgtaca gagggaagaa gcgtcatgag atgcctccac acatctatgc
     1981 tatatctgaa tctgcttaca gatgcatgct tcaagatcgt gaggaccagt caattctttg
     2041 cacgggtgag tcaggtgctg ggaagacaga aaatacaaag aaagttattc agtaccttgc
     2101 ccatgttgct tcttcacata aaggaagaaa ggaccataat attcctgggg aacttgaacg
     2161 gcagcttttg caagcaaatc caattctgga atcatttgga aatgcgaaga ctgtgaaaaa
     2221 tgataactca tctcgttttg gcaaatttat tcggatcaac tttgatgtaa ctggctatat
     2281 cgttggggcc aacattgaaa cataccttct ggaaaagtct cgtgctgttc gtcaagcaaa
     2341 agatgaacgt acttttcata tcttttacca gttgttatct ggagcaggag aacacctaaa
     2401 gtctgatttg cttcttgaag gatttaataa ctacaggttt ctctccaatg gctatattcc
     2461 tattccggga cagcaagaca aagataattt ccaggagacc atggaagcaa tgcacataat
     2521 gggcttctcc catgaagaga ttctgtcaat gcttaaagta gtatcttcag tgctacagtt
     2581 tggaaatatt tctttcaaaa aggagagaaa tactgatcaa gcttccatgc cagaaaatac
     2641 agttgcgcag aagctctgcc atcttcttgg gatgaatgtg atggagttta ctcgggccat
     2701 cctgactccc cggatcaagg tcggccgaga ctatgtgcaa aaagcccaga ccaaagaaca
     2761 ggcagatttt gcagtagaag cattggcaaa agctacctat gagcggctct ttcgctggct
     2821 cgttcatcgc atcaataaag ctctggatag gaccaaacgt cagggagcat ctttcattgg
     2881 aatcctggat attgctggat ttgaaatttt tgagctgaac tcctttgaac aactttgcat
     2941 caactacacc aatgagaagc tgcagcagct gttcaaccac accatgttta tcctagaaca
     3001 agaggaatac cagcgcgaag gcatcgagtg gaacttcatc gatttcgggc tggatctgca
     3061 gccatgcatc gacctaatag agagacctgc gaaccctcct ggtgtactgg cccttttgga
     3121 tgaagaatgc tggttcccta aagccacaga taaaaccttt gttgaaaaac tggttcaaga
     3181 gcaaggttcc cactccaagt ttcagaaacc tcgacaatta aaagacaaag ctgatttttg
     3241 cattatacat tatgcaggga aggtggacta taaggcagat gagtggctga tgaagaatat
     3301 ggaccccctg aatgacaacg tggccaccct tttgcaccag tcatcagaca gatttgtggc
     3361 agagctttgg aaagatgtgg accgtatcgt gggtctggat caagtcactg gtatgactga
     3421 gacagctttt ggctccgcat ataaaaccaa gaagggcatg tttcgtaccg ttgggcaact
     3481 ctacaaagaa tctctcacca agctgatggc aactctccga aacaccaacc ctaactttgt
     3541 tcgttgtatc attccaaatc acgagaagag ggctggaaaa ttggatccac acctagtcct
     3601 agatcagctt cgctgtaatg gtgtcctgga agggatccga atctgtcgcc agggcttccc
     3661 taaccgaata gttttccagg aattcagaca gagatatgag atcctaactc caaatgctat
     3721 tcctaaaggt tttatggatg gtaaacaggc ctgtgaacga atgatccggg ctttagaatt
     3781 ggacccaaac ttgtacagaa ttggacagag caagatattt ttcagagctg gagttctggc
     3841 acacttagag gaagaaagag atttaaaaat caccgatatc attatcttct tccaggccgt
     3901 ttgcagaggt tacctggcca gaaaggcctt tgccaagaag cagcagcaac taagtgcctt
     3961 aaaggtcttg cagcggaact gtgccgcgta cctgaaatta cggcactggc agtggtggcg
     4021 agtcttcaca aaggtgaagc cgcttctaca agtgactcgc caggaggaag aacttcaggc
     4081 caaagatgaa gagctgttga aggtgaagga gaagcagaca aaggtggaag gagagctgga
     4141 ggagatggag cggaagcacc agcagctttt agaagagaag aatatccttg cagaacaact
     4201 acaagcagag actgagctct ttgctgaagc agaagagatg agggcaagac ttgctgctaa
     4261 aaagcaggaa ttagaagaga ttctacatga cttggagtct agggttgaag aagaagaaga
     4321 aagaaaccaa atcctccaaa atgaaaagaa aaaaatgcaa gcacatattc aggacctgga
     4381 agaacagcta gacgaggagg aaggggctcg gcaaaagctg cagctggaaa aggtgacagc
     4441 agaggccaag atcaagaaga tggaagagga gattctgctt ctcgaggacc aaaattccaa
     4501 gttcatcaaa gaaaagaaac tcatggaaga tcgcattgct gagtgttcct ctcagctggc
     4561 tgaagaggaa gaaaaggcga aaaacttggc caaaatcagg aataagcaag aagtgatgat
     4621 ctcagatcta gaagaacgct taaagaaaga agaaaagact cgtcaggaac tggaaaaggc
     4681 caaaagaaaa ctcgacgggg agacgaccga cctgcaggac cagatcgcag agctgcaggc
     4741 gcagattgat gagctcaagc tgcagctggc caagaaggag gaggagctgc agggcgcact
     4801 ggccagaggt gatgatgaaa cactccataa gaacaatgcc cttaaagttg tgcgagagct
     4861 acaagcccaa attgctgaac ttcaggaaga ctttgaatcc gagaaggctt cacggaacaa
     4921 ggccgaaaag cagaaaaggg acttgagtga ggaactggaa gctctgaaaa cagagctgga
     4981 ggacacgctg gacaccacgg cagcccagca ggaactacgt acaaaacgtg aacaagaagt
     5041 ggcagagctg aagaaagctc ttgaggagga aactaagaac catgaagctc aaatccagga
     5101 catgagacaa agacacgcaa cagccctgga ggagctctca gagcagctgg aacaggccaa
     5161 gcggttcaaa gcaaatctag agaagaacaa gcagggcctg gagacagata acaaggagct
     5221 ggcgtgtgag gtgaaggtcc tgcagcaggt caaggctgag tctgagcaca agaggaagaa
     5281 gctcgacgcg caggtccagg agctccatgc caaggtctct gaaggcgaca ggctcagggt
     5341 ggagctggcg gagaaagcaa gtaagctgca gaatgagcta gataatgtct ccacccttct
     5401 ggaagaagca gagaagaagg gtattaaatt tgctaaggat gcagctagtc ttgagtctca
     5461 actacaggat acacaggagc ttcttcagga ggagacacgc cagaaactaa acctgagcag
     5521 tcggatccgg cagctggaag aggagaagaa cagtcttcag gagcagcagg aggaggagga
     5581 ggaggccagg aagaacctgg agaagcaagt gctggccctg cagtcccagt tggctgatac
     5641 caagaagaaa gtagatgacg acctgggaac aattgaaagt ctggaagaag ccaagaagaa
     5701 gcttctgaag gacgcggagg ccctgagcca gcgcctggag gagaaggcac tggcgtatga
     5761 caaactggag aagaccaaga accgcctgca gcaggagctg gacgacctca cggtggacct
     5821 ggaccaccag cgccaggtcg cctccaactt ggagaagaag cagaagaagt ttgaccagct
     5881 gttagcagaa gagaagagca tctctgctcg ctatgccgaa gagcgggacc gggccgaagc
     5941 cgaggccaga gagaaagaaa ccaaagccct gtcactggcc cgggccctcg aggaagccct
     6001 ggaggccaag gaggagtttg agaggcagaa caagcagctc cgagcagaca tggaagacct
     6061 catgagctcc aaagatgatg tgggaaaaaa cgttcacgaa cttgaaaaat ccaaacgggc
     6121 cctagagcag caggtggagg aaatgaggac ccagctggag gagctggaag acgaactcca
     6181 ggccacggaa gatgccaagc ttcgtctgga ggtcaacatg caggccatga aggcgcagtt
     6241 cgagagagac ctgcaaacca gggatgagca gaatgaagag aagaagcggc tgctgatcaa
     6301 acaggtgcgg gagctcgagg cggagctgga ggatgagagg aaacagcggg cgcttgctgt
     6361 agcttcaaag aaaaagatgg agatagacct gaaggacctc gaagcccaaa tcgaggctgc
     6421 gaacaaagct cgggatgagg tgattaagca gctccgcaag ctccaggctc agatgaagga
     6481 ttaccaacgt gaattagaag aagctcgtgc atccagagat gagatttttg ctcaatccaa
     6541 agagagtgaa aagaaattga agagtctgga agcagaaatc cttcaattgc aggaggaact
     6601 tgcctcatct gagcgagccc gccgacacgc cgagcaggag agagatgagc tggcggacga
     6661 gatcaccaac agcgcctctg gcaagtccgc gctgctggat gagaagcggc gtctggaagc
     6721 tcggatcgca cagctggagg aggagctgga agaggagcag agcaacatgg agctgctcaa
     6781 cgaccgcttc cgcaagacca ctctacaggt ggacacactg aacgccgagc tagcagccga
     6841 gcgcagcgcc gcccagaaga gtgacaatgc acgccagcaa ctggagcggc agaacaagga
     6901 gctgaaggcc aagctgcagg aactcgaggg tgctgtcaag tctaagttca aggccaccat
     6961 ctcagccctg gaggccaaga ttgggcagct ggaggagcag cttgagcagg aagccaagga
     7021 acgagcagcc gccaacaaat tagtccgtcg cactgagaag aagctgaaag aaatcttcat
     7081 gcaggttgag gatgagcgtc gacacgcgga ccagtataaa gagcagatgg agaaggccaa
     7141 cgctcggatg aagcagctta aacgccagct ggaggaagca gaagaagaag cgacgcgtgc
     7201 caacgcatct cggcgtaaac tccagcggga actggatgat gccaccgagg ccaacgaggg
     7261 cctgagccgc gaggtcagca ccctgaagaa ccggctgagg cggggtggcc ccatcagctt
     7321 ctcttccagc cgatctggcc ggcgccagct gcaccttgaa ggagcttccc tggagctctc
     7381 cgacgatgac acagaaagta agaccagtga tgtcaacgag acgcagccac cccagtcaga
     7441 gtaaggtacc gcgggcccgg gatccaccgg atctagataa ctgatca
//
