LOCUS       sequence-89255-e        7476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7476)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MyosinIIB-N-18
FEATURES             Location/Qualifiers
     source          1..7476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6027..6036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6033..7463
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgccacc atggcgcaga
       61 gaactggact cgaggatcca gagaggtatc tctttgtgga cagggctgtc atctacaacc
      121 ctgccactca agctgattgg acagctaaaa agctagtgtg gattccatca gaacgccatg
      181 gttttgaggc agctagtatc aaagaagaac ggggagatga agttatggtg gagttggcag
      241 agaatggaaa gaaagcaatg gtcaacaaag atgatattca gaagatgaac ccacctaagt
      301 tttccaaggt ggaggatatg gcagaattga catgcttgaa tgaagcttcc gttttacata
      361 atctgaagga tcgctactat tcaggactaa tctatactta ttctggactc ttctgtgtag
      421 ttataaaccc ttacaagaat cttccaattt actctgagaa tattattgaa atgtacagag
      481 ggaagaagcg tcatgagatg cctccacaca tctatgctat atctgaatct gcttacagat
      541 gcatgcttca agatcgtgag gaccagtcaa ttctttgcac gggtgagtca ggtgctggga
      601 agacagaaaa tacaaagaaa gttattcagt accttgccca tgttgcttct tcacataaag
      661 gaagaaagga ccataatatt cctggggaac ttgaacggca gcttttgcaa gcaaatccaa
      721 ttctggaatc atttggaaat gcgaagactg tgaaaaatga taactcatct cgttttggca
      781 aatttattcg gatcaacttt gatgtaactg gctatatcgt tggggccaac attgaaacat
      841 accttctgga aaagtctcgt gctgttcgtc aagcaaaaga tgaacgtact tttcatatct
      901 tttaccagtt gttatctgga gcaggagaac acctaaagtc tgatttgctt cttgaaggat
      961 ttaataacta caggtttctc tccaatggct atattcctat tccgggacag caagacaaag
     1021 ataatttcca ggagaccatg gaagcaatgc acataatggg cttctcccat gaagagattc
     1081 tgtcaatgct taaagtagta tcttcagtgc tacagtttgg aaatatttct ttcaaaaagg
     1141 agagaaatac tgatcaagct tccatgccag aaaatacagt tgcgcagaag ctctgccatc
     1201 ttcttgggat gaatgtgatg gagtttactc gggccatcct gactccccgg atcaaggtcg
     1261 gccgagacta tgtgcaaaaa gcccagacca aagaacaggc agattttgca gtagaagcat
     1321 tggcaaaagc tacctatgag cggctctttc gctggctcgt tcatcgcatc aataaagctc
     1381 tggataggac caaacgtcag ggagcatctt tcattggaat cctggatatt gctggatttg
     1441 aaatttttga gctgaactcc tttgaacaac tttgcatcaa ctacaccaat gagaagctgc
     1501 agcagctgtt caaccacacc atgtttatcc tagaacaaga ggaataccag cgcgaaggca
     1561 tcgagtggaa cttcatcgat ttcgggctgg atctgcagcc atgcatcgac ctaatagaga
     1621 gacctgcgaa ccctcctggt gtactggccc ttttggatga agaatgctgg ttccctaaag
     1681 ccacagataa aacctttgtt gaaaaactgg ttcaagagca aggttcccac tccaagtttc
     1741 agaaacctcg acaattaaaa gacaaagctg atttttgcat tatacattat gcagggaagg
     1801 tggactataa ggcagatgag tggctgatga agaatatgga ccccctgaat gacaacgtgg
     1861 ccaccctttt gcaccagtca tcagacagat ttgtggcaga gctttggaaa gatgtggacc
     1921 gtatcgtggg tctggatcaa gtcactggta tgactgagac agcttttggc tccgcatata
     1981 aaaccaagaa gggcatgttt cgtaccgttg ggcaactcta caaagaatct ctcaccaagc
     2041 tgatggcaac tctccgaaac accaacccta actttgttcg ttgtatcatt ccaaatcacg
     2101 agaagagggc tggaaaattg gatccacacc tagtcctaga tcagcttcgc tgtaatggtg
     2161 tcctggaagg gatccgaatc tgtcgccagg gcttccctaa ccgaatagtt ttccaggaat
     2221 tcagacagag atatgagatc ctaactccaa atgctattcc taaaggtttt atggatggta
     2281 aacaggcctg tgaacgaatg atccgggctt tagaattgga cccaaacttg tacagaattg
     2341 gacagagcaa gatatttttc agagctggag ttctggcaca cttagaggaa gaaagagatt
     2401 taaaaatcac cgatatcatt atcttcttcc aggccgtttg cagaggttac ctggccagaa
     2461 aggcctttgc caagaagcag cagcaactaa gtgccttaaa ggtcttgcag cggaactgtg
     2521 ccgcgtacct gaaattacgg cactggcagt ggtggcgagt cttcacaaag gtgaagccgc
     2581 ttctacaagt gactcgccag gaggaagaac ttcaggccaa agatgaagag ctgttgaagg
     2641 tgaaggagaa gcagacaaag gtggaaggag agctggagga gatggagcgg aagcaccagc
     2701 agcttttaga agagaagaat atccttgcag aacaactaca agcagagact gagctctttg
     2761 ctgaagcaga agagatgagg gcaagacttg ctgctaaaaa gcaggaatta gaagagattc
     2821 tacatgactt ggagtctagg gttgaagaag aagaagaaag aaaccaaatc ctccaaaatg
     2881 aaaagaaaaa aatgcaagca catattcagg acctggaaga acagctagac gaggaggaag
     2941 gggctcggca aaagctgcag ctggaaaagg tgacagcaga ggccaagatc aagaagatgg
     3001 aagaggagat tctgcttctc gaggaccaaa attccaagtt catcaaagaa aagaaactca
     3061 tggaagatcg cattgctgag tgttcctctc agctggctga agaggaagaa aaggcgaaaa
     3121 acttggccaa aatcaggaat aagcaagaag tgatgatctc agatctagaa gaacgcttaa
     3181 agaaggaaga aaagactcgt caggaactgg aaaaggccaa aagaaaactc gacggggaga
     3241 cgaccgacct gcaggaccag atcgcagagc tgcaggcgca gattgatgag ctcaagctgc
     3301 agctggccaa gaaggaggag gagctgcagg gcgcactggc cagaggtgat gatgaaacac
     3361 tccataagaa caatgccctt aaagttgtgc gagagctaca agcccaaatt gctgaacttc
     3421 aggaagactt tgaatccgag aaggcttcac ggaacaaggc cgaaaagcag aaaagggact
     3481 tgagtgagga actggaagct ctgaaaacag agctggagga cacgctggac accacggcag
     3541 cccagcagga actacgtaca aaacgtgaac aagaagtggc agagctgaag aaagctcttg
     3601 aggaggaaac taagaaccat gaagctcaaa tccaggacat gagacaaaga cacgcaacag
     3661 ccctggagga gctctcagag cagctggaac aggccaagcg gttcaaagca aatctagaga
     3721 agaacaagca gggcctggag acagataaca aggagctggc gtgtgaggtg aaggtcctgc
     3781 agcaggtcaa ggctgagtct gagcacaaga ggaagaagct cgacgcgcag gtccaggagc
     3841 tccatgccaa ggtctctgaa ggcgacaggc tcagggtgga gctggcggag aaagcaagta
     3901 agctgcagaa tgagctagat aatgtctcca cccttctgga agaagcagag aagaagggta
     3961 ttaaatttgc taaggatgca gctagtcttg agtctcaact acaggataca caggagcttc
     4021 ttcaggagga gacacgccag aaactaaacc tgagcagtcg gatccggcag ctggaagagg
     4081 agaagaacag tcttcaggag cagcaggagg aggaggagga ggccaggaag aacctggaga
     4141 agcaagtgct ggccctgcag tcccagttgg ctgataccaa gaagaaagta gatgacgacc
     4201 tgggaacaat tgaaagtctg gaagaagcca agaagaagct tctgaaggac gcggaggccc
     4261 tgagccagcg cctggaggag aaggcactgg cgtatgacaa actggagaag accaagaacc
     4321 gcctgcagca ggagctggac gacctcacgg tggacctgga ccaccagcgc caggtcgcct
     4381 ccaacttgga gaagaagcag aagaagtttg accagctgtt agcagaagag aagagcatct
     4441 ctgctcgcta tgccgaagag cgggaccggg ccgaagccga ggccagagag aaagaaacca
     4501 aagccctgtc actggcccgg gccctcgagg aagccctgga ggccaaggag gagtttgaga
     4561 ggcagaacaa gcagctccga gcagacatgg aagacctcat gagctccaaa gatgatgtgg
     4621 gaaaaaacgt tcacgaactt gaaaaatcca aacgggccct agagcagcag gtggaggaaa
     4681 tgaggaccca gctggaggag ctggaagacg aactccaggc cacggaagat gccaagcttc
     4741 gtctggaggt caacatgcag gccatgaagg cgcagttcga gagagacctg caaaccaggg
     4801 atgagcagaa tgaagagaag aagcggctgc tgatcaaaca ggtgcgggag ctcgaggcgg
     4861 agctggagga tgagaggaaa cagcgggcgc ttgctgtagc ttcaaagaaa aagatggaga
     4921 tagacctgaa ggacctcgaa gcccaaatcg aggctgcgaa caaagctcgg gatgaggtga
     4981 ttaagcagct ccgcaagctc caggctcaga tgaaggatta ccaacgtgaa ttagaagaag
     5041 ctcgtgcatc cagagatgag atttttgctc aatccaaaga gagtgaaaag aaattgaaga
     5101 gtctggaagc agaaatcctt caattgcagg aggaacttgc ctcatctgag cgagcccgcc
     5161 gacacgccga gcaggagaga gatgagctgg cggacgagat caccaacagc gcctctggca
     5221 agtccgcgct gctggatgag aagcggcgtc tggaagctcg gatcgcacag ctggaggagg
     5281 agctggaaga ggagcagagc aacatggagc tgctcaacga ccgcttccgc aagaccactc
     5341 tacaggtgga cacactgaac gccgagctag cagccgagcg cagcgccgcc cagaagagtg
     5401 acaatgcacg ccagcaactg gagcggcaga acaaggagct gaaggccaag ctgcaggaac
     5461 tcgagggtgc tgtcaagtct aagttcaagg ccaccatctc agccctggag gccaagattg
     5521 ggcagctgga ggagcagctt gagcaggaag ccaaggaacg agcagccgcc aacaaattag
     5581 tccgtcgcac tgagaagaag ctgaaagaaa tcttcatgca ggttgaggat gagcgtcgac
     5641 acgcggacca gtataaagag cagatggaga aggccaacgc tcggatgaag cagcttaaac
     5701 gccagctgga ggaagcagaa gaagaagcga cgcgtgccaa cgcatctcgg cgtaaactcc
     5761 agcgggaact ggatgatgcc accgaggcca acgagggcct gagccgcgag gtcagcaccc
     5821 tgaagaaccg gctgaggcgg ggtggcccca tcagcttctc ttccagccga tctggccggc
     5881 gccagctgca ccttgaagga gcttccctgg agctctccga cgatgacaca gaaagtaaga
     5941 ccagtgatgt caacgagacg cagccacccc agtcagaggg cagcggtgga ggcagcgcat
     6001 ccggcggaag cggaagcgga ccggtcgcca ccatggtgag caagggcgag gaggtcatca
     6061 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     6121 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     6181 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     6241 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     6301 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     6361 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     6421 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     6481 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     6541 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     6601 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     6661 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
     6721 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
     6781 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     6841 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     6901 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     6961 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     7021 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     7081 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     7141 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     7201 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     7261 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     7321 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     7381 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     7441 gcatggacga gctgtacaag taagcggccg cgactc
//
