LOCUS       sequence-89256-e        7574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7574)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-MyosinIIC-N-18
FEATURES             Location/Qualifiers
     source          1..7574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6087..6096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6093..7523
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gaaaggatag tgacgtcaga tccgctagca ccgccaccat ggctgcagtg accatgtccg
       61 tgtctgggag gaaggtagcc tccaggccag gcccggtgcc tgaggcagcc caatcgttcc
      121 tctacgcgcc ccggacgcca aatgtaggtg gccctggagg gccacaggtg gagtggacag
      181 cccggcgcat ggtgtgggtg ccctcggaac tgcatgggtt cgaggcagca gccctgcggg
      241 atgaagggga ggaggaggca gaagtggagc tggcggagag tgggcgccgc ctgcggctgc
      301 ccagggacca gatccagcgc atgaacccac ccaagttcag caaggcagaa gatatggctg
      361 agctcacctg cctcaacgag gcctcggtcc tgcacaacct gcgagaacgc tactactccg
      421 ggctcattta tacctactct ggcctcttct gtgtggtcat taacccatac aagcagctgc
      481 ccatctacac ggaggccatt gttgaaatgt accggggcaa gaagcgccat gaggtgccac
      541 ctcacgtgta tgctgtgacg gagggcgcgt accgcagcat gcttcaggat cgtgaggatc
      601 aatccattct ctgcacggga gagtctggcg ctgggaagac ggagaacacc aagaaggtca
      661 tccagtacct ggcccatgtg gcatcatctc caaagggcag gaaggagcct ggtgtccctg
      721 cctccgtcag caccatgtct tatggggagc tagagcgtca gcttcttcaa gccaacccca
      781 tcctagaggc ctttggcaat gccaagacag tgaagaacga caactcttcc cgatttggca
      841 aattcatccg catcaacttt gatattgctg gctacatcgt gggagcaaac atcgagacct
      901 atctgttgga gaagtcccgg gccatcagac aggccaagga tgaatgcagc ttccatatct
      961 tctaccagct gctagggggc gctggggagc agctaaaagc tgacctcctt ctggagccct
     1021 gttcccatta tcgcttcctg accaatgggc cctcatcgtc cccgggccag gagcgtgagt
     1081 tattccagga gaccctggag tccctgcgtg tgctgggcct cctcccagaa gagatcactg
     1141 ccatgctgcg cactgtctct gctgtcctcc agtttggcaa cattgtcctg aagaaagagc
     1201 gcaatacgga ccaagccacc atgcctgaca acacagctgc ccagaagctt tgccgcctct
     1261 tgggactcgg agtgaccgac ttctccagag cccttctcac accccgcatc aaagtgggcc
     1321 gagattatgt tcagaaagca caaaccaagg agcaggctga ctttgcgctg gaggctctgg
     1381 ccaaagctac ctatgagcgc ctgttccgct ggctggttct gcggctcaac cgtgccctgg
     1441 acagaagccc gcggcagggt gcctccttcc tgggcatcct ggacatcgcg ggctttgaga
     1501 tcttccagct gaactccttc gagcagctgt gcatcaacta caccaacgag aagctacagc
     1561 agctattcaa ccacaccatg ttcgtgctgg agcaggagga gtaccagcga gagggcatcc
     1621 cctggacctt cctagacttc gggttggacc tgcaaccttg catcgacctc attgagcgtc
     1681 cggccaaccc tccaggtctc ctggccctgc tggacgagga gtgctggttc cccaaggcca
     1741 cggacaagtc ttttgtggag aaggtcgccc aggagcaggg cagccacccc aaattccagc
     1801 gccccaggaa cctgcgagat caggccgact tcagcgtcct gcactacgcc ggcaaggttg
     1861 actacaaagc cagtgagtgg ctgatgaaga acatggaccc actgaatgac aatgtggccg
     1921 ccttgcttca ccagagcacg gatcgtctca cagctgagat ctggaaggat gtggagggca
     1981 tcgtggggct ggagcaagta agcagccttg gagatggccc accgggaggc cgcccccgcc
     2041 gtggaatgtt tcggactgtg gggcagctct acaaagaatc cctgagccgc ctcatggcca
     2101 cgctcagcaa caccaaccct agttttgtcc gctgcatcgt tcccaatcat gagaagaggg
     2161 ctggaaagct ggagccgcgc ctggtgctgg accaactccg ttgtaacggg gtcctcgagg
     2221 gtatacgcat ctgtcgccaa ggcttcccca accgcatcct cttccaggag ttccgacagc
     2281 gctatgaaat cctcaccccg aacgctattc ccaagggctt catggacggc aaacaggcct
     2341 gtgagaagat gatccaggcc ctggagctag accccaacct gtaccgtgtt ggccaaagca
     2401 agatcttctt ccgggcaggg gtcctggccc agctggagga ggagcgggac ctgaaagtca
     2461 ccgacatcat agtgtctttc caggcagcgg cacggggcta cctggcccgt agggctttcc
     2521 agagacggca gcagcagcag agtgctctga gggtgatgca gagaaactgt gctgcctacc
     2581 tcaagctcag gaactggcag tggtggaggc tgttcatcaa ggtgaagccc ctgctgcagg
     2641 tgacacggca ggatgaggtg ctgcaggcgc gcgcccagga gctgcagaaa gttcaggagc
     2701 tgcagcagca gagcgctcgt gaagtggggg aactgcaggg tcgagtggca cagctagagg
     2761 aggagcgcac gcgcctggct gagcagcttc gagcagaagc cgagctctgc tctgaggccg
     2821 aggagacgcg ggcgcgactg gctgcccgga agcaggagct ggagctggtg gtgacagagc
     2881 tggaggcacg agtgggcgag gaagaagagt gcagccggca gctgcagagt gagaagaaga
     2941 ggctgcagca gcatatccag gagctagaga gccacctgga agctgaggag ggtgcccggc
     3001 agaagctaca gctggagaag gtgaccacag aggccaagat gaagaaattt gaggaggacc
     3061 tgctgctcct ggaggaccag aattccaagc tgagcaagga gcggaggctg ctggaggagc
     3121 ggctggctga gttctcctca caggcagcag aagaggaaga gaaagtcaaa agtctcaaca
     3181 agctgaggct caaatatgaa gccacaatct cagacatgga agaccggctg aagaaggagg
     3241 agaagggacg ccaggaacta gagaagctga agcgacggct ggacggggag agctcagagc
     3301 ttcaggagca gatggtggag cagaagcaga gggcagagga actgctcgca cagctgggcc
     3361 gcaaggagga tgagctgcag gccgccctgc tcagggcaga ggaagagggt ggtgcccgtg
     3421 cccagttgct caagtccctg cgagaggcac aggctggcct tgctgaggct caggaggacc
     3481 tggaagctga gcgggtagcc agggccaagg cggagaagca gcgccgggac ctgggcgagg
     3541 agttggaggc cctacgtggg gagctcgagg acactctgga ttccaccaac gcccagcagg
     3601 agctgcggtc caagagggag caggaggtga cagagctgaa gaaagcattg gaagaggagt
     3661 cccgtgccca tgaggtgtcc atgcaggagc tgagacagag gcatagccag gcactggtgg
     3721 agatggccga gcagttggag caagcccgga ggggcaaagg tgtgtgggag aagactcggc
     3781 tatccctgga ggctgaggtg tccgagctga aggccgagct gagcagcctg cagacctcga
     3841 gacaggaggg tgagcagaag aggcgccgcc tggagtccca gctacaggag gtccagggcc
     3901 gatccagtga ttcggagcgg gctcggtctg aggctgctga gaagctgcag agagcccagg
     3961 cggaacttga gagcgtgtcc acagccctga gtgaggcgga gtccaaagcc atcaggctgg
     4021 gcaaggagct gagcagtgca gagtcccagc tgcatgacac ccaggaactg cttcaggagg
     4081 agaccagggc aaagctggcc ttggggtccc gtgtgcgtgc cctagaggcc gaggcggcgg
     4141 ggcttcggga gcagatggaa gaggaggtgg ttgccaggga acgggctggc cgggagctgc
     4201 agagcacgca ggcccagctc tctgaatggc ggcgccgcca ggaagaagag gctgcggtgc
     4261 tggaggctgg ggaggaggct cggcgccgtg cagcccggga ggcagagacc ctgacccagc
     4321 gcctggcaga aaagactgag gctgtagaac gactggagcg agcccggcgc cgactgcagc
     4381 aggagttgga cgatgccact gtggatctgg ggcagcagaa gcagctcctg agcacactgg
     4441 agaagaagca gcggaaattt gaccagctcc tggcagagga gaaggctgca gttctacggg
     4501 ctgtggaaga ccgtgaacgg atagaggccg aaggccggga gcgagaggcc cgggccctgt
     4561 cgctgacccg ggccctggaa gaggagcagg aggcccggga ggagctggag aggcagaacc
     4621 gtgctctgag ggctgagctg gaagcactgc tgagcagcaa ggatgacgtg ggcaagaacg
     4681 tgcacgagct ggagcgagcc cgtaaggcgg ctgaacaggc agccagtgac ctgcggacac
     4741 aggtgacaga attggaggat gagctgacag ccgcagagga tgccaagctg cgcctggagg
     4801 tgactgtgca ggctctgaag gctcaacatg aacgcgacct gcagggccgc gatgatgccg
     4861 gtgaggagag gcggaggcag ctggccaagc agctaagaga cgcagaggta gagcgcgatg
     4921 aggaacggaa gcagagggca ctggctatgg ctgcccgcaa gaagctggag ctggaactgg
     4981 aggagttgaa ggcgcagaca tctgctgctg ggcagggcaa ggaagaggca gtgaagcagc
     5041 tgaagaagat gcaggtccag atgaaggagc tgtggcggga ggtagaggag acgcgtagct
     5101 cccgcgacga gatgtttacc ctgagcaggg aaaatgagaa gaagctcaag gggctggaag
     5161 ctgaggtgct gcgtctgcaa gaggaacttg ctgcctcaga ccgagcccgg aggcaggccc
     5221 agcaagacag agacgagatg gcagaggagg tggccagtgg caatcttagc aaggcagcca
     5281 ccctggagga aaaacggcag ctggaggggc gactgagcca gttggaagag gagctggagg
     5341 aagaacagaa caactcggag ctgctcaagg accattaccg aaagctagtg ctacaggtcg
     5401 agtccctcac cacagaactg tctgccgaac gaagtttctc agccaaggcc gagagtggac
     5461 ggcagcagct ggagcggcag atccaggaac tgcgggcccg cttgggtgaa gaggatgctg
     5521 gagcccgagc caggcagaaa atgctgatcg ctgctctgga gtctaaactg gcccaggcag
     5581 aggagcagct ggagcaggag agcagggagc gcatcctctc tggcaagctg gtacgcagag
     5641 ctgagaagcg gctgaaggag gtagttcttc aggtggatga agagcgcagg gtggctgacc
     5701 aggtccggga ccagctggag aaaagcaacc tccggctgaa gcagctcaag aggcagctgg
     5761 aggaggcaga ggaggaggca tctcgggcac aggctggtcg gaggcggctg cagcgggagc
     5821 tggaggacgt cactgagtct gcagaatcca tgaaccggga ggtgaccacg ctgaggaaca
     5881 ggctccggcg tggcccactt acattcacca cacggactgt gcgccaggtg ttccggctgg
     5941 aagagggcgt ggcttctgac gaggaagagg ctgaaggagc tgaacctggc tctgcaccag
     6001 gccaggagcc ggaggctccg ccccctgcca caccccaaag cagcggtgga gcaagcgcag
     6061 ccagtggtag cgcggatcca ccggtcgcca ccatggtgag caagggcgag gaggtcatca
     6121 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     6181 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     6241 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     6301 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     6361 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     6421 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     6481 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     6541 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     6601 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     6661 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     6721 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
     6781 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
     6841 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     6901 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     6961 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     7021 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     7081 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     7141 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     7201 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     7261 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     7321 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     7381 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     7441 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     7501 gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag ccataccaca
     7561 tgtagaaatt ttct
//
