LOCUS       sequence-89257-e        3045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3045)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..1462
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggtcat
       61 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
      721 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
      781 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
      841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      901 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
      961 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     1021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     1081 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     1141 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1201 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     1261 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     1321 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     1381 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     1441 cggcatggac gagctgtaca agtccggaag tgctgggagc gcaagcggaa gcggaggctc
     1501 cggttttaac tacgaacgtc caaaacactt catccagtcc caaaacccat gtggctccag
     1561 attgcagcct cctggaccag aaacctccag cttctctagc cagaccaaac agtcttccat
     1621 tatcatccag ccccgccagt gtacagagca aagattttct gcctcctcaa cactgagctc
     1681 tcacatcacc atgtcctcct ctgctttccc tgcttctccc cagcagcatg ctggctccaa
     1741 cccaggccaa agggttacaa ccacctataa ccagtcccca gccagcttcc tcagctccat
     1801 attaccatca cagcctgatt acaatagcag taaaatccct tccgctatgg attccaacta
     1861 tcaacagtcc tcagctggcc aacctataaa tgcaaagcca tcccaaactg caaatgctaa
     1921 gcccatacca agaactcctg atcatgaaat acaaggatca aaagaagctt tgattcaaga
     1981 tttggaaaga aagctgaaat gcaaggacac ccttcttcat aatggaaatc aacgtctaac
     2041 atatgaagag aagatggctc gcagattgct aggaccacag aatgcagctg ctgtgtttca
     2101 agctcaggat gacagtggtg cacaagactc gcagcaacac aactcagaac atgcgcgact
     2161 gcaagttcct acatcacaag taagaagtag atcaacctca aggggagatg tgaatgatca
     2221 ggatgcaatc caggagaaat tttacccacc acgtttcatt caagtgccag agaacatgtc
     2281 gattgatgaa ggaagattct gcagaatgga cttcaaagtg agtggactgc cagctcctga
     2341 tgtgtcatgg tatctaaatg gaagaacagt tcaatcagat gatttgcaca aaatgatagt
     2401 gtctgagaag ggtcttcatt cactcatctt tgaagtagtc agagcttcag atgcaggggc
     2461 ttatgcatgt gttgccaaga atagagcagg agaagccacc ttcactgtgc agctggatgt
     2521 ccttgcaaaa gaacataaaa gagcaccaat gtttatctac aaaccacaga gcaaaaaagt
     2581 tttagaggga gattcagtga aactagaatg ccagatctcg gctatacctc caccaaagct
     2641 tttctggaaa agaaataatg aaatggtaca attcaacact gaccgaataa gcttatatca
     2701 agataacact ggaagagtta ctttactgat aaaagatgta aacaagaaag atgctgggtg
     2761 gtatactgtg tcagcagtta atgaagctgg agtgactaca tgtaacacaa gattagacgt
     2821 tacggcacgt ccaaaccaaa ctcttccagc tcctaagcag ttacgggttc gaccaacatt
     2881 cagcaaatat ttagcactta atgggaaagg tttgaatgta aaacaagctt ttaacccaga
     2941 aggagaattt cagcgtttgg cagctcaatc tggactctat gaaagtgaag aactttaagg
     3001 atccaccgga tctagataac tgatcataat cagccatacc acatt
//
