LOCUS       sequence-89260-e        1728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1728)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..1728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    189..241
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      249..258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             255..1685
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc tgcatcactg
       61 ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc aagcagctca
      121 acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc tttctcaaac
      181 tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      241 caccggtcgc caccatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca
      301 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
      361 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
      421 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
      481 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
      541 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
      601 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
      661 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
      721 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
      781 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
      841 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
      901 agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg
      961 gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc
     1021 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     1081 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     1141 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     1201 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     1261 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     1321 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     1381 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     1441 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     1501 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     1561 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     1621 agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca
     1681 agtaagcggc cgcgactcta gatcataatc agccatacca cattgaga
//
