LOCUS       sequence-89262-e        3350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3350)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..3350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1875..1884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1881..3311
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgcc accatgagtg ctgcagtgac tgcagggaag ctggcacggg
       61 caccggccga ccctgggaaa gccggggtcc ccggagttgc agctcccgga gctccggcgg
      121 cggctccacc ggcgaaagag atcccggagg tcctagtgga cccacgcagc cggcggcgct
      181 atgtgcgggg ccgctttttg ggcaagggcg gctttgccaa gtgcttcgag atctcggacg
      241 cggacaccaa ggaggtgttc gcgggcaaga ttgtgcctaa gtctctgctg ctcaagccgc
      301 accagaggga gaagatgtcc atggaaatat ccattcaccg cagcctcgcc caccagcacg
      361 tcgtaggatt ccacggcttt ttcgaggaca acgacttcgt gttcgtggtg ttggagctct
      421 gccgccggag gtctctcctg gagctgcaca agaggaggaa agccctgact gagcctgagg
      481 cccgatacta cctacggcaa attgtgcttg gctgccagta cctgcaccga aaccgagtta
      541 ttcatcgaga cctcaagctg ggcaaccttt tcctgaatga agatctggag gtgaaaatag
      601 gggattttgg actggcaacc aaagtcgaat atgacgggga gaggaagaag accctgtgtg
      661 ggactcctaa ttacatagct cccgaggtgc tgagcaagaa agggcacagt ttcgaggtgg
      721 atgtgtggtc cattgggtgt atcatgtata ccttgttagt gggcaaacca ccttttgaga
      781 cttcttgcct aaaagagacc tacctccgga tcaagaagaa tgaatacagt attcccaagc
      841 acatcaaccc cgtggccgcc tccctcatcc agaagatgct tcagacagat cccactgccc
      901 gcccaaccat taacgagctg cttaatgacg agttctttac ttctggctat atccctgccc
      961 gtctccccat cacctgcctg accattccac caaggttttc gattgctccc agcagcctgg
     1021 accccagcaa ccggaagccc ctcacagtcc tcaataaagg cttggagaac cccctgcctg
     1081 agcgtccccg ggaaaaagaa gaaccagtgg ttcgagagac aggtgaggtg gtcgactgcc
     1141 acctcagtga catgctgcag cagctgcaca gtgtcaatgc ctccaagccc tcggagcgtg
     1201 ggctggtcag gcaagaggag gctgaggatc ctgcctgcat ccccatcttc tgggtcagca
     1261 agtgggtgga ctattcggac aagtacggcc ttgggtatca gctctgtgat aacagcgtgg
     1321 gggtgctctt caatgactca acacgcctca tcctctacaa tgatggtgac agcctgcagt
     1381 acatagagcg tgacggcact gagtcctacc tcaccgtgag ttcccatccc aactccttga
     1441 tgaagaagat caccctcctt aaatatttcc gcaattacat gagcgagcac ttgctgaagg
     1501 caggtgccaa catcacgccg cgcgaaggtg atgagctcgc ccggctgccc tacctacgga
     1561 cctggttccg cacccgcagc gccatcatcc tgcacctcag caacggcagc gtgcagatca
     1621 acttcttcca ggatcacacc aagctcatct tgtgcccact gatggcagcc gtgacctaca
     1681 tcgacgagaa gcgggacttc cgcacatacc gcctgagtct cctggaggag tacggctgct
     1741 gcaaggagct ggccagccgg ctccgctacg cccgcactat ggtggacaag ctgctgagct
     1801 cacgctcggc cagcaaccgt ctcaaggcct ccggtagcgg cagcgcatcc ggcggaagcg
     1861 gaagcggacc ggtcgccacc atggtgagca agggcgagga ggtcatcaaa gagttcatgc
     1921 gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg
     1981 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc
     2041 ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg
     2101 tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt
     2161 gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc
     2221 tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg
     2281 gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc
     2341 gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact
     2401 acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact
     2461 actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
     2521 agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc ggcagcaccg
     2581 gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc atcaaagagt
     2641 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     2701 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     2761 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     2821 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     2881 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     2941 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     3001 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     3061 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     3121 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     3181 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     3241 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc atggacgagc
     3301 tgtacaagta agcggccgcg actctagatc ataatcagcc ataccacatt
//
