LOCUS       sequence-89263-e        2175 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2175)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..2175
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..1459
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg aggtcatcaa
       61 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      121 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      181 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
      241 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
      301 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
      361 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
      421 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
      481 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
      541 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
      601 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
      661 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
      721 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
      781 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
      841 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      901 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
      961 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     1021 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     1081 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     1141 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     1201 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     1261 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     1321 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     1381 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     1441 catggacgag ctgtacaagt ccggactcag atctcgagcc atggctagtc gaggcgcaac
     1501 aagacccaac gggccaaata ctggaaataa aatatgccag ttcaaactag tacttctggg
     1561 agagtctgct gttggcaaat caagcctagt gcttcgtttt gtgaagggcc aatttcatga
     1621 atttcaagag agtaccatag gggctgcttt tctaacccaa actgtgtgtc ttgatgatac
     1681 aacagtaaag tttgaaatat gggatacagc tggtcaagaa cgataccata gcttagcacc
     1741 aatgtactac agaggagcac aagcagccat agttgtatat gatatcacaa atgaggagtc
     1801 ctttgccaga gccaaaaact gggttaaaga acttcagagg caagccagtc ctaacattgt
     1861 aatagcttta tcaggaaaca aggctgatct tgcaaataaa agagctgtcg atttccagga
     1921 agcacagtcc tatgcagatg acaacagttt attattcatg gagacatcag ctaaaacatc
     1981 gatgaatgta aatgaaatat tcatggcaat agctaaaaag ttgccaaaga acgaaccaca
     2041 gaatccagga gcaaattctg ccagaggaag aggagtagac cttactgaac ccacgcagcc
     2101 aaccaggaat cagtgttgta gtaactaagg atccaccgga tctagataac tgatcataat
     2161 cagccatacc acatt
//
