LOCUS       sequence-89264-e        2151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2151)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..2151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..1462
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggtcat
       61 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
      721 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
      781 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
      841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      901 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
      961 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     1021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     1081 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     1141 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1201 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     1261 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     1321 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     1381 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     1441 cggcatggac gagctgtaca agtccggact cagatctcga gccatgacct ctaggaagaa
     1501 agtgttgctg aaggttatca tcctgggaga ttctggagtc gggaagacat cactcatgaa
     1561 ccagtatgtg aataagaaat tcagcaatca gtacaaagcc acaataggag ctgactttct
     1621 gaccaaggag gtgatggtgg atgacaggct agtcacaatg cagatatggg acacagcagg
     1681 acaggaacgg ttccagtctc tcggtgtggc cttctacaga ggtgcagact gctgcgttct
     1741 ggtatttgat gtgactgccc ccaacacatt caaaacccta gatagctgga gagatgagtt
     1801 tctcatccag gccagtcccc gagatcctga aaacttccca tttgttgtgt tgggaaacaa
     1861 gattgacctc gaaaacagac aagtggccac aaagcgggca caggcctggt gctacagcaa
     1921 aaacaacatt ccctactttg agaccagtgc caaggaggcc atcaacgtgg agcaggcgtt
     1981 ccagacgatt gcacggaatg cacttaagca ggaaacggag gtggagctgt acaacgaatt
     2041 tcctgaacct atcaaactgg acaagaatga ccgggccaag gcctcggcag aaagctgcag
     2101 ttgctgagga tccaccggat ctagataact gatcataatc agccatacca c
//
