LOCUS       sequence-89266-e        1692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1692)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..1692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      217..226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             223..1653
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 aatccgctag cgctaccgga ctcagatctc gagctcgcca ccatgattca caccaacctg
       61 aagaaaaagt tcagctgctg cgtcctggtc tttcttctgt ttgcagtcat ctgtgtgtgg
      121 aaggaaaaga agaaagggag ttactatgat tcctttaaat tgcaaaccaa ggaattcctg
      181 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      241 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
      301 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      361 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
      421 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
      481 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
      541 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
      601 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      661 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
      721 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
      781 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
      841 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
      901 gggcatggca ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac
      961 aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     1021 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1081 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     1141 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     1201 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     1261 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1321 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1381 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     1441 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     1501 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     1561 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     1621 ttcctgtacg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     1681 ccataccaca tg
//
