LOCUS       sequence-89269-e        7881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7881)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Talin-R-15
FEATURES             Location/Qualifiers
     source          1..7881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..1455
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggaggt catcaaagag
       61 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
      121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      181 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
      241 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
      301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
      361 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
      421 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
      481 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
      541 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
      601 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
      661 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc
      721 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc
      781 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
      841 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      901 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
      961 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     1021 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     1081 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     1141 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     1201 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     1261 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     1321 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     1381 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     1441 gacgagctgt acaagtccgg actcagatct cgagctcaag ctagcttcga attcgccacc
     1501 atgcagcagt acaaccgagt ggggaaagtg gagcacggct ctgtggctct gccagccatc
     1561 atgcgctctg gagcctctgg tcctgagaat ttccaggtgg ggagcatgcc acctgcccag
     1621 cagcagatca ccagtggcca gatgcaccga ggacacatgc cacctctgac ttcagcccag
     1681 caggcgctca ctggaaccat taactccagc atgcaggctg tgcaggctgc ccaggccact
     1741 ctggatgact ttgagactct accccctctt ggccaggatg ctgcctccaa agcttggcgt
     1801 aagaacaaga tggatgaatc aaagcatgag atccactccc aggtagatgc catcacagct
     1861 ggcactgcct ctgtggtaaa cctgacagca ggagatcctg cagagacaga ctatacagca
     1921 gtgggctgtg cagtcaccac catctcttcc aacctgacgg agatgtcccg tggggtgaag
     1981 ctattggctg ccctgctgga ggatgaaggc ggcaatggcc ggccccttct gcaagcagca
     2041 aagggccttg caggggctgt gtcggaactg cttcgcagtg ctcagcccgc cagcgctgag
     2101 cctcgtcaga acctgctgca ggcagccggg aacgtgggcc aggccagtgg ggagctgttg
     2161 cagcaaattg gggaaagtga cactgacccc cacttccagg atgttctaat gcagctagca
     2221 aacgcagtgg caagtgctgc agccgccctg gtcctcaagg ccaagagtgt ggcccagcgc
     2281 acagaggatt ccgggcttca gacccaagtg attgctgcag ctacgcaatg tgctctgtcc
     2341 acttcccagc tggtggcctg taccaaggtg gtagcaccta caatcagctc acctgtgtgc
     2401 caagaacagc tagtcgaggc tgggcgactg gtggccaaag ctgtggaggg ctgtgtgtct
     2461 gcctcgcaag cagctacaga agatggacag ctgcttcgag gggtaggagc agcagccacg
     2521 gctgtcaccc aggccctcaa tgagctgctg cagcacgtga aggcccacgc caccggagct
     2581 gggcctgccg gtcggtatga ccaagccact gataccatcc tcaccgtcac tgaaaacatc
     2641 ttcagctcca tgggtgatgc tggggagatg gtgcgccagg cccgcatcct ggcccaagcc
     2701 acctcagacc tggtcaatgc tatcaaggcc gatgctgagg gggagagtga tctggagaac
     2761 tctagaaagc tcctgagtgc tgccaagatc ctcgctgatg ccaccgccaa gatggtggag
     2821 gcggccaagg gagcagccgc ccaccctgac agtgaggaac agcagcagcg actgcgtgaa
     2881 gcagctgagg ggcttcgcat ggccaccaat gcagctgcgc agaacgccat caagaagaag
     2941 ttggtgcagc gcctggagca tgcagccaag caagctgcag cctctgcaac acagaccatt
     3001 gctgcagccc aacatgcagc ctctgccccc aaggcctctg cgggccccca gcccctactg
     3061 gtgcagagct gtaaggccgt ggcagagcag attccgttgc tggtgcaggg tgtccgagga
     3121 agtcaagctc agcctgacag ccctagtgct cagctcgccc tcattgctgc cagccagagc
     3181 ttcctgcagc caggtgggaa gatggtggca gcggcaaaag cctcagtgcc aacaattcag
     3241 gaccaggctt cagcgatgca gctgagtcag tgcgcaaaga acctaggcac tgccctggcc
     3301 gagctgcgca ctgctgctca gaaggctcag gaggcatgtg gacctttgga gatggattct
     3361 gcactgagtg ttgtacaaaa tctagagaaa gacctacagg aaataaaagc agcagctcga
     3421 gatggcaagc ttaagccctt acctggggaa acaatggaga agtgtaccca agatcttggc
     3481 aacagcacca aagcggtgag ctctgccatc gccaagctgc tgggggagat tgcccagggc
     3541 aatgagaact atgcaggtat tgcagctcgg gatgtagctg gtggactaag gtcactagcc
     3601 caggctgcgc ggggtgtggc tgcgctgaca tcagatcctg cagtgcaggc cattgtgctt
     3661 gacacagcca gcgacgttct ggacaaggcc agcagcctca ttgaagaggc aaaaaaagca
     3721 tctggacacc caggggaccc agaaagccag caaaggcttg ctcaggtagc taaagcagtg
     3781 acccaagccc tgaaccgctg tgtcagctgc ctgcctggcc aaagagacgt ggataatgcc
     3841 ctacgagccg ttggagatgc cagcaagcga ctcctgagtg acttgcttcc tccaagcacg
     3901 gggacatttc aagaagcaca gagccgattg aatgaagctg cagctgggtt aaatcaggcc
     3961 gccacagaac tggtgcaagc ctcccgagga actcctcagg acctggctcg ggcctccggc
     4021 cgatttggac aggacttcag caccttcctg gaagctggtg tggagatggc cggacaggcc
     4081 ccgagccagg aggaccgagc ccaggtggtg tccaacctga agggcatatc catgtcttca
     4141 agcaaacttc tccttgccgc taaggccttg tccacagacc ctgcttctcc caacctcaag
     4201 agtcagctgg ctgcggctgc ccgggcagtg acggacagca tcaaccagct catcaccatg
     4261 tgcacccagc aggcacctgg ccagaaggag tgtgacaatg cacttcggca gctagagaca
     4321 gtccgagaac tcctggagaa tccagtccag cccatcaacg acatgtccta cttcggttgc
     4381 ttggacagtg tcatggagaa ctccaaggtc ctaggtgagg ccatgactgg catctcccaa
     4441 aatgccaaga atggaaatct gccggagttt ggagatgcca ttgccacagc ctccaaggct
     4501 ctctgtggct tcacggaggc agccgcacag gcagcatatc tagttggtgt ctctgacccc
     4561 aacagccaag ctggacagca aggactggtg gaacccacac agtttgcccg tgcaaaccag
     4621 gcaattcaga tggcctgtca gagtctgggg gagcctggct gtacccaggc ccaggtgtta
     4681 tctgcagcca ctattgtagc caaacacaca tctgcattgt gtaacagctg tcgcctggct
     4741 tccgctagga ctgccaatcc cactgccaag cgccagtttg tacagtcagc caaggaggtg
     4801 gccaacagta cagccaatct tgtcaagacc atcaaggcac tagatgggga cttcacagaa
     4861 gagaaccgtg cccagtgccg agcagccaca gcccctctgc tggaagctgt ggataacctg
     4921 agtgcctttg cctccaaccc tgagttctcc agcgtccctg cccagatcag ccctgagggc
     4981 agagcagcca tggagcccat tgtaatctct gctaagacaa tgttggagag tgctggaggg
     5041 ctcatccaga cagctcgggc gttagcagtc aatccccgag accccccacg ttggtctgtg
     5101 ctagctggcc actctcggac tgtctcagat tccatcaaga aacttattac aagcatgagg
     5161 gacaaagccc cagggcagct ggagtgtgag acagccattg cggctctgaa cagctgcttg
     5221 cgggacctag atcaggcttc gcttgccgct gtcagccagc agcttgctcc ccgtgaagga
     5281 atctctcaag aggctttgca cacccagatg ctcactgcag tgcaagaaat ttctcatctc
     5341 attgagccgc tggccagcgc tgctcgggct gaagcctccc agctgggaca caaggtgtcc
     5401 caaatggccc agtactttga accactcacc ctggctgcag tgggtgctgc gtctaagacc
     5461 ctgagccacc cgcagcagat ggcacttctg gaccagacta aaacgttggc agagtctgcc
     5521 ttgcagttgc tatatactgc caaggaggct ggtggtaacc ccaagcaagc agcacacacc
     5581 caggaagccc tggaggaggc tgtgcagatg atgacagagg ccgtagaaga cctgacgacg
     5641 accctcaacg aagcagccag tgctgcagga gtcgttggcg gcatggtgga ctctatcact
     5701 caggccatca accagctaga tgaaggacct atgggtgacc cagaaggctc attcgtagat
     5761 taccagacaa ccatggtgag gacagccaag gccattgctg tcactgttca ggagatggta
     5821 accaagtcaa acaccagccc tgaagagcta ggccctcttg ccaaccagct gaccagtgac
     5881 tatggccgac tggcctcaca agccaagcct gcagctgtgg ctgctgaaaa tgaagagata
     5941 ggcgctcaca tcaaacaccg agtacaggag ctgggccatg gctgctctgc tctggtcacc
     6001 aaggcaggtg ccttgcagtg tagccccagt gatgtctaca ccaagaagga gctcatagag
     6061 tgtgcccgca gagtgtcaga gaaggtctcc catgttctgg ctgcactcca ggctgggaat
     6121 cgtggtaccc aggcctgcat tacagcagcc agtgctgtgt ctggtatcat tgctgacctc
     6181 gacaccacca tcatgttcgc tactgctggc acacttaacc gtgagggtgc tgaaactttt
     6241 gctgaccacc gggagggtat cttaaagaca gcaaaggttc ttgtggagga caccaaggtc
     6301 ctagtgcaga atgcagctgg gagccaggag aagttggcac aagccgccca gtcctccgtg
     6361 gccaccatta cccgcctcgc tgatgtggtc aagctcggtg cagccagcct aggagccgaa
     6421 gaccctgaaa ctcaggtggt gctgatcaat gcagtaaagg acgtagccaa ggccctgggt
     6481 gacctcatca gcgctacgaa ggctgcagcg ggcaaagttg gggatgaccc tgcagtgtgg
     6541 cagctcaaga actctgccaa ggtgatggtg accaatgtga catcattgct caagacagtg
     6601 aaggctgtgg aagatgaggc caccaaaggc acacgggccc tagagaagac ctctacccct
     6661 gaagacttca tccgaatgac caagggtatt actatggcaa cagccaaagc cgttgctgct
     6721 ggcaattcct gtcgacagga agatgtcatt gccacagcca atctgagccg acgggctatt
     6781 gcggacatgc ttcgggcttg caaggaagca gctttccacc cagaagtggc gcctgatgta
     6841 cggctccgag ccctgcacta tggccgggag tgtgccaatg gttacctgga actgctggac
     6901 cacgtgctgc tgacccttca gaagccaaac ccagacctga agcagcagct aactggacac
     6961 tcaaagcggg ttgctggctc tgtgactgag ctcatccagg ctgctgaagc catgaaagga
     7021 acagagtggg tggacccaga ggaccctact gtcattgctg agaatgagct cttgggagcc
     7081 gcagccgcca tcgaggctgc agccaagaag ctggagcagc tgaagcccag ggccaaaccc
     7141 aaggaggcgg atgagtcctt gaactttgag gaacaaatcc tagaagctgc caagtccatc
     7201 gctgcagcca ccagtgcact ggtaaaggct gcgtcagcag cccagaggga gctggtggct
     7261 caaggaaagg tgggcgccat tccagccaat gcactggatg atgggcagtg gtcgcagggc
     7321 ctcatttccg ctgcccgtat ggtggctgcg gccaccaaca atctgtgtga agcagctaac
     7381 gcagctgtcc agggccatgc tagccaggag aaactcattt cctcagccaa gcaagtagct
     7441 gcctccacag ctcagctcct ggtagcttgc aaggtcaaag cagatcagga ctctgaggca
     7501 atgaaacggc tccaggctgc tggcaatgca gtgaagaggg cctcagataa cctggtaaag
     7561 gcggcccaga aagctgcagc cttcgaagac caggagaatg agacggtggt ggtgaaggag
     7621 aaaatggttg ggggcattgc ccagattatc gcagcacagg aagagatgct tcggaaggaa
     7681 cgagagctgg aagaggctcg gaaaaagctc gcccagatcc ggcagcagca gtacaagttc
     7741 ttgccttcag agcttcgaga cgagcactaa aaaagccctg tgtatttaat gcagacccag
     7801 cccagagact gtgcctgcca ctaccaaagc cttctgggct gctcaggacc caagctgccc
     7861 aaccccagca ctcccccaaa g
//
