LOCUS       sequence-89271-e        1953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 554, 
            Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1953)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      478..487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             484..1914
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 agatccgcta gcgccaccat ggtgggtcgg aacagcgcca tcgccgccgg tgtatgcggg
       61 gcccttttca ttgggtactg catctacttc gaccgcaaaa gacgaagtga ccccaacttc
      121 aagaacaggc ttcgagaacg aagaaagaaa cagaagcttg ccaaggagag agctgggctt
      181 tccaagttac ctgaccttaa agatgctgaa gctgttcaga agttcttcct tgaagaaata
      241 cagcttggtg aagagttact agctcaaggt gaatatgaga agggcgtaga ccatctgaca
      301 aatgcaattg ctgtgtgtgg acagccacag cagttactgc aggtcttaca gcaaactctt
      361 ccaccaccag tgttccagat gcttctgact aagctcccaa caattagtca gagaattgta
      421 agtgctcaga gcttggctga agatgatgtg gaaggcggta gcggggatcc accggtcgcc
      481 accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg
      541 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
      601 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
      661 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
      721 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
      781 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
      841 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
      901 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
      961 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     1021 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     1081 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     1141 ggccgccacc acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc
     1201 accgcctcct ccgaggacaa caacatggcc gtcatcaaag agttcatgcg cttcaaggtg
     1261 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1321 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     1381 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     1441 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1501 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     1561 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     1621 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     1681 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     1741 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     1801 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     1861 tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa gtaagcggcc
     1921 gcgactctag atcataatca gccataccac att
//
