LOCUS       sequence-89272-e        3770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3770)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-TPX2-N-10
FEATURES             Location/Qualifiers
     source          1..3770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2296..2305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2302..3732
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 ctagttacgt cagatccgct agccgccacc atgtcacaag ttaaaagctc ttattcctat
       61 gatgccccct cggatttcat caatttttca tccttggatg atgaaggaga tactcaaaac
      121 atagattcat ggtttgagga gaaggccaat ttggagaata agttactggg gaagaatgga
      181 actggagggc tttttcaggg caaaactcct ttgagaaagg ctaatcttca gcaagctatt
      241 gtcacacctt tgaaaccagt tgacaacact tactacaaag aggcagaaaa agaaaatctt
      301 gtggaacaat ccattccgtc aaatgcttgt tcttccctgg aagttgaggc agccatatca
      361 agaaaaactc cagcccagcc tcagagaaga tctcttaggc tttctgctca gaaggatttg
      421 gaacagaaag aaaagcatca tgtaaaaatg aaagccaaga gatgtgccac tcctgtaatc
      481 atcgatgaaa ttctaccctc taagaaaatg aaagtttcta acaacaaaaa gaagccagag
      541 gaagaaggca gtgctcatca agatactgct gaaaagaatg catcttcccc agagaaagcc
      601 aagggtagac atactgtgcc ttgtatgcca cctgcaaagc agaagtttct aaaaagtact
      661 gaggagcaag agctggagaa gagtatgaaa atgcagcaag aggtggtgga gatgcggaaa
      721 aagaatgaag aattcaagaa acttgctctg gctggaatag ggcaacctgt gaagaaatca
      781 gtgagccagg tcaccaaatc agttgacttc cacttccgca cagatgagcg aatcaaacaa
      841 catcctaaga accaggagga atataaggaa gtgaacttta catctgaact acgaaagcat
      901 ccttcatctc ctgcccgagt gactaaggga tgtaccattg ttaagccttt caacctgtcc
      961 caaggaaaga aaagaacatt tgatgaaaca gtttctacat atgtgcccct tgcacagcaa
     1021 gttgaagact tccataaacg aacccctaac agatatcatt tgaggagcaa gaaggatgat
     1081 attaacctgt taccctccaa atcttctgtg accaagattt gcagagaccc acagactcct
     1141 gtactgcaaa ccaaacaccg tgcacgggct gtgacctgca aaagtacagc agagctggag
     1201 gctgaggagc tcgagaaatt gcaacaatac aaattcaaag cacgtgaact tgatcccaga
     1261 atacttgaag gtgggcccat cttgcccaag aaaccacctg tgaaaccacc caccgagcct
     1321 attggctttg atttggaaat tgagaaaaga atccaggagc gagaatcaaa gaagaaaaca
     1381 gaggatgaac actttgaatt tcattccaga ccttgcccta ctaagatttt ggaagatgtt
     1441 gtgggtgttc ctgaaaagaa ggtacttcca atcaccgtcc ccaagtcacc agcctttgca
     1501 ttgaagaaca gaattcgaat gcccaccaaa gaagatgagg aagaggacga accggtagtg
     1561 ataaaagctc aacctgtgcc acattatggg gtgcctttta agccccaaat cccagaggca
     1621 agaactgtgg aaatatgccc tttctcgttt gattctcgag acaaagaacg tcagttacag
     1681 aaggagaaga aaataaaaga actgcagaaa ggggaggtgc ccaagttcaa ggcacttccc
     1741 ttgcctcatt ttgacaccat taacctgcca gagaagaagg taaagaatgt gacccagatt
     1801 gaacctttct gcttggagac tgacagaaga ggtgctctga aggcacagac ttggaagcac
     1861 cagctggaag aagaactgag acagcagaaa gaagcagctt gtttcaaggc tcgtccaaac
     1921 accgtcatct ctcaggagcc ctttgttccc aagaaagaga agaaatcagt tgctgagggc
     1981 ctttctggtt ctctagttca ggaacctttt cagctggcta ctgagaagag agccaaagag
     2041 cggcaggagc tggagaagag aatggctgag gtagaagccc agaaagccca gcagttggag
     2101 gaggccagac tacaggagga agagcagaaa aaagaggagc tggccaggct acggagagaa
     2161 ctggtgcata aggcaaatcc aatacgcaag taccagggtc tggagataaa gtcaagtgac
     2221 cagcctctga ctgtgcctgt atctcccaaa ttctccactc gattccactg ctcgggaagc
     2281 acggatccac cggtcgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg
     2341 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     2401 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     2461 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     2521 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     2581 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     2641 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     2701 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     2761 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     2821 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     2881 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     2941 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc
     3001 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt catcaaagag
     3061 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
     3121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3181 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
     3241 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
     3301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
     3361 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
     3421 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     3481 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     3541 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
     3601 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     3661 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag
     3721 ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat
//
