LOCUS       sequence-89273-e        2884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2884)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..1462
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggtcat
       61 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
      721 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
      781 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
      841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      901 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
      961 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     1021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     1081 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     1141 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1201 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     1261 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     1321 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     1381 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     1441 cggcatggac gagctgtaca agtccggact cagatctcga gtgcgtgagt gcatctccat
     1501 ccacgttggc caggctggtg tccagattgg caatgcctgc tgggagctct actgcctgga
     1561 acacggcatc cagcccgatg gccagatgcc aagtgacaag accattgggg gaggagatga
     1621 ctccttcaac accttcttca gtgagacggg cgctggcaag cacgtgcccc gggctgtgtt
     1681 tgtagacttg gaacccacag tcattgatga agttcgcact ggcacctacc gccagctctt
     1741 ccaccctgag cagctcatca caggcaagga agatgctgcc aataactatg cccgagggca
     1801 ctacaccatt ggcaaggaga tcattgacct tgtgttggac cgaattcgca agctggctga
     1861 ccagtgcacc ggtcttcagg gcttcttggt tttccacagc tttggtgggg gaactggttc
     1921 tgggttcacc tccctgctca tggaacgtct ctcagttgat tatggcaaga agtccaagct
     1981 ggagttctcc atttacccag caccccaggt ttccacagct gtagttgagc cctacaactc
     2041 catcctcacc acccacacca ccctggagca ctctgattgt gccttcatgg tagacaatga
     2101 ggccatctat gacatctgtc gtagaaacct cgatatcgag cgcccaacct acactaacct
     2161 taaccgcctt attagccaga ttgtgtcctc catcactgct tccctgagat ttgatggagc
     2221 cctgaatgtt gacctgacag aattccagac caacctggtg ccctaccccc gcatccactt
     2281 ccctctggcc acatatgccc ctgtcatctc tgctgagaaa gcctaccatg aacagctttc
     2341 tgtagcagag atcaccaatg cttgctttga gccagccaac cagatggtga aatgtgaccc
     2401 tcgccatggt aaatacatgg cttgctgcct gttgtaccgt ggtgacgtgg ttcccaaaga
     2461 tgtcaatgct gccattgcca ccatcaaaac caagcgcagc atccagtttg tggattggtg
     2521 ccccactggc ttcaaggttg gcatcaacta ccagcctccc actgtggtgc ctggtggaga
     2581 cctggccaag gtacagagag ctgtgtgcat gctgagcaac accacagcca ttgctgaggc
     2641 ctgggctcgc ctggaccaca agtttgacct gatgtatgcc aagcgtgcct ttgttcactg
     2701 gtacgtgggt gaggggatgg aggaaggcga gttttcagag gcccgtgaag atatggctgc
     2761 ccttgagaag gattatgagg aggttggtgt ggattctgtt gaaggagagg gtgaggaaga
     2821 aggagaggaa tactaaggat ccaccggatc tagataactg atcataatca gccataccac
     2881 attg
//
