LOCUS       sequence-89275-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-VASP-5
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..1462
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggtcat
       61 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      241 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      421 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      541 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      601 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg
      721 caccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc
      781 cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
      841 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      901 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
      961 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
     1021 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
     1081 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
     1141 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1201 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
     1261 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
     1321 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
     1381 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta
     1441 cggcatggac gagctgtaca agtccggact cagatctatg agcgagacgg tcatctgttc
     1501 cagccgggct actgtgatgc tttatgatga cagcaacaag cgatggctcc ctgctggcac
     1561 tggtccccag gccttcagcc gcgtccagat ataccacaac cccactgcta actccttccg
     1621 agttgttggc cgcaagatgc agccggatca gcaggtggtt atcaactgtg ccatcattcg
     1681 gggtgtcaag tacaatcagg ccactcccat cttccatcag tggcgagatg cccgccaggt
     1741 ctggggcctc aacttcggca gcaaggagga cgccatacag tttgcaacag gcatggccaa
     1801 cgccctagag gccttggaag gaggtgggcc tcccccagcc ccagcacccc ctgcctggtc
     1861 tgcccagaat ggtccctccc cagaggagct ggaacaacag aaaaggcagc cggagcatat
     1921 ggagcgccgg gtctccaatg caggaggccc acctgctccc ccagctgggg gccctcctcc
     1981 acctccagga cctccccctc ctccaggtcc ccccccaccc ccaggtctgc cctcctcagg
     2041 ggtatctggg gcaggtcatg gagcaggggc agccccaccc cctgcacccc cactccctac
     2101 aacacagggc cccaatagtg ggggttccgg ggccccaggc ctggctgctg ccattgctgg
     2161 agccaaactc aggaaagtga gcaagcagga ggaggcctct ggggggcccc tggcccccaa
     2221 agctgagaac agtcgaagca ctggtggggg gcttatggaa gagatgaacg ccatgctggc
     2281 ccggagaaga aaagccacac aggttgggga gaagcccccc aaagacgagt cagccagtga
     2341 ggagtcagag gcccgactcc ctgcccagag tgaacctgtg agaagaccct gggagaagaa
     2401 cagcacaacc ttgccaagga tgaagtcgtc ctcttctgtg actacctccg aggcccaccc
     2461 ctcgacgccc tgctccagtg atgactccga cttggagagg gtgaagcagg agcttctgga
     2521 agaggtgcgg aaggagctac agaaaatgaa agaggaaatc atcgaagtct ttgtccagga
     2581 gctgaggaag cggggttctc cttgagcggc cgcgactcta gatcata
//
