LOCUS       sequence-89276-e        3878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3878)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-VE-Cadherin-N-10
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2070..2081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2403..2412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2409..3839
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gaccgtcaga tccgctagcc gccaccatgc agaggctcat gatgctcctc gccacatcgg
       61 gcgcctgcct gggcctgctg gcagtggcag cagtggcagc agcaggtgct aaccctgccc
      121 aacgggacac ccacagcctg ctgcccaccc accggcgcca aaagagagat tggatttgga
      181 accagatgca cattgatgaa gagaaaaaca cctcacttcc ccatcatgta ggcaagatca
      241 agtcaagcgt gagtcgcaag aatgccaagt acctgctcaa aggagaatat gtgggcaagg
      301 tcttccgggt cgatgcagag acaggagacg tgttcgccat tgagaggctg gaccgggaga
      361 atatctcaga gtaccacctc actgctgtca ttgtggacaa ggacactggt gaaaacctgg
      421 agactccttc cagcttcacc atcaaagttc atgacgtgaa cgacaactgg cctgtgttca
      481 cgcatcggtt gttcaatgcg tccgtgcctg agtcgtcggc tgtggggacc tcagtcatct
      541 ctgtgacagc agtggatgca gacgacccca ctgtgggaga ccacgcctct gtcatgtacc
      601 aaatcctgaa ggggaaagag tattttgcca tcgataattc tggacgtatt atcacaataa
      661 cgaaaagctt ggaccgagag aagcaggcca ggtatgagat cgtggtggaa gcgcgagatg
      721 cccagggcct ccggggggac tcgggcacgg ccaccgtgct ggtcactctg caagacatca
      781 atgacaactt ccccttcttc acccagacca agtacacatt tgtcgtgcct gaagacaccc
      841 gtgtgggcac ctctgtgggc tctctgtttg ttgaggaccc agatgagccc cagaaccgga
      901 tgaccaagta cagcatcttg cggggcgact accaggacgc tttcaccatt gagacaaacc
      961 ccgcccacaa cgagggcatc atcaagccca tgaagcctct ggattatgaa tacatccagc
     1021 aatacagctt catcgtcgag gccacagacc ccaccatcga cctccgatac atgagccctc
     1081 ccgcgggaaa cagagcccag gtcattatca acatcacaga tgtggacgag ccccccattt
     1141 tccagcagcc tttctaccac ttccagctga aggaaaacca gaagaagcct ctgattggca
     1201 cagtgctggc catggaccct gatgcggcta ggcatagcat tggatactcc atccgcagga
     1261 ccagtgacaa gggccagttc ttccgagtca caaaaaaggg ggacatttac aatgagaaag
     1321 aactggacag agaagtctac ccctggtata acctgactgt ggaggccaaa gaactggatt
     1381 ccactggaac ccccacagga aaagaatcca ttgtgcaagt ccacattgaa gttttggatg
     1441 agaatgacaa tgccccggag tttgccaagc cctaccagcc caaagtgtgt gagaacgctg
     1501 tccatggcca gctggtcctg cagatctccg caacagacaa ggacataaca ccacgaaacg
     1561 tgaagttcaa attcactttg aatactgaga acaactttac cctcacggat aatcacgata
     1621 acacggccaa catcacagtc aagtatgggc agtttgaccg ggagcatacc aaggtccact
     1681 tcctacccgt ggtcatctca gacaatggga tgccaagtcg cacgggcacc agcacgctga
     1741 ccgtggccgt gtgcaagtgc aacgagcagg gcgagttcac cttctgcgag gatatggccg
     1801 cccaggtggg cgtgagcatc caggcagtgg tagccatctt actctgcatc ctcaccatca
     1861 cagtgatcac cctgctcatc ttcctgcggc ggcggctccg gaagcaggcc cgcgcgcacg
     1921 gcaagagcgt gccggagatc cacgagcagc tggtcaccta cgacgaggag ggcggcggcg
     1981 agatggacac caccagctac gatgtgtcgg tgctcaactc ggtgcgccgc ggcggggcca
     2041 agcccccgcg gcccgcgctg gacgcccggc cttccctcta tgcgcaggtg cagaagccac
     2101 cgaggcacgc gcctggggca cacggagggc ccggggagat ggcagccatg atcgaggtga
     2161 agaaggacga ggcggaccac gacggcgacg gcccccccta cgacacgctg cacatctacg
     2221 gctacgaggg ctccgagtcc atagccgagt ccctcagctc cctgggcacc gactcatccg
     2281 actctgacgt ggattacgac ttccttaacg actggggacc caggtttaag atgctggctg
     2341 agctgtacgg ctcggacccc cgggaggagc tgctgtattc gggaagcacg gatccaccgg
     2401 tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     2461 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     2521 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     2581 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     2641 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2701 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     2761 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     2821 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     2881 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     2941 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     3001 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     3061 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     3121 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     3181 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     3241 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     3301 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     3361 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     3421 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     3481 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     3541 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     3601 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     3661 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     3721 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     3781 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtaag
     3841 cggccgcgac tctagatcat aatcagccat accacatt
//
