LOCUS       sequence-89277-e        2952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 554, Emission: 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2952)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1473..1482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1479..2909
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 cgacgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccca
       61 tgtccaccag gtccgtgtcc tcgtcctcct accgcaggat gttcggcggc ccgggcaccg
      121 cgagccggcc gagctccagc cggagctacg tgactacgtc cacccgcacc tacagcctgg
      181 gcagcgcgct gcgccccagc accagccgca gcctctacgc ctcgtccccg ggcggcgtgt
      241 atgccacgcg ctcctctgcc gtgcgcctgc ggagcagcgt gcccggggtg cggctcctgc
      301 aggactcggt ggacttctcg ctggccgacg ccatcaacac cgagttcaag aacacccgca
      361 ccaacgagaa ggtggagctg caggagctga atgaccgctt cgccaactac atcgacaagg
      421 tgcgcttcct ggagcagcag aataagatcc tgctggccga gctcgagcag ctcaagggcc
      481 aaggcaagtc gcgcctgggg gacctctacg aggaggagat gcgggagctg cgccggcagg
      541 tggaccagct aaccaacgac aaagcccgcg tcgaggtgga gcgcgacaac ctggccgagg
      601 acatcatgcg cctccgggag aaattgcagg aggagatgct tcagagagag gaagccgaaa
      661 acaccctgca atctttcaga caggatgttg acaatgcgtc tctggcacgt cttgaccttg
      721 aacgcaaagt ggaatctttg caagaagaga ttgccttttt gaagaaactc cacgaagagg
      781 aaatccagga gctgcaggct cagattcagg aacagcatgt ccaaatcgat gtggatgttt
      841 ccaagcctga cctcacggct gccctgcgtg acgtacgtca gcaatatgaa agtgtggctg
      901 ccaagaacct gcaggaggca gaagaatggt acaaatccaa gtttgctgac ctctctgagg
      961 ctgccaaccg gaacaatgac gccctgcgcc aggcaaagca ggagtccact gagtaccgga
     1021 gacaggtgca gtccctcacc tgtgaagtgg atgcccttaa aggaaccaat gagtccctgg
     1081 aacgccagat gcgtgaaatg gaagagaact ttgccgttga agctgctaac taccaagaca
     1141 ctattggccg cctgcaggat gagattcaga atatgaagga ggaaatggct cgtcaccttc
     1201 gtgaatacca agacctgctc aatgttaaga tggcccttga cattgagatt gccacctaca
     1261 ggaagctgct ggaaggcgag gagagcagga tttctctgcc tcttccaaac ttttcctccc
     1321 tgaacctgag ggaaactaat ctggattcac tccctctggt tgatacccac tcaaaaagga
     1381 cacttctgat taagacggtt gaaactagag atggacaggt tatcaacgaa acttctcagc
     1441 atcacgatga ccttgaaggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg
     1501 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     1561 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     1621 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     1681 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     1741 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     1801 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     1861 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     1921 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     1981 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     2041 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     2101 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctggggc
     2161 atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag gacaacaaca
     2221 tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
     2281 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     2341 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
     2401 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
     2461 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
     2521 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
     2581 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2641 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
     2701 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
     2761 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
     2821 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
     2881 tgtacggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat
     2941 accacattga ga
//
