LOCUS       sequence-89278-e        2943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 554, Emission: 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2943)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Vimentin-N-18
FEATURES             Location/Qualifiers
     source          1..2943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1471..1480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1477..2907
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgtcc accaggtccg tgtcctcgtc ctcctaccgc
       61 aggatgttcg gcggcccggg caccgcgagc cggccgagct ccagccggag ctacgtgact
      121 acgtccaccc gcacctacag cctgggcagc gcgctgcgcc ccagcaccag ccgcagcctc
      181 tacgcctcgt ccccgggcgg cgtgtatgcc acgcgctcct ctgccgtgcg cctgcggagc
      241 agcgtgcccg gggtgcggct cctgcaggac tcggtggact tctcgctggc cgacgccatc
      301 aacaccgagt tcaagaacac ccgcaccaac gagaaggtgg agctgcagga gctgaatgac
      361 cgcttcgcca actacatcga caaggtgcgc ttcctggagc agcagaataa gatcctgctg
      421 gccgagctcg agcagctcaa gggccaaggc aagtcgcgcc tgggggacct ctacgaggag
      481 gagatgcggg agctgcgccg gcaggtggac cagctaacca acgacaaagc ccgcgtcgag
      541 gtggagcgcg acaacctggc cgaggacatc atgcgcctcc gggagaaatt gcaggaggag
      601 atgcttcaga gagaggaagc cgaaaacacc ctgcaatctt tcagacagga tgttgacaat
      661 gcgtctctgg cacgtcttga ccttgaacgc aaagtggaat ctttgcaaga agagattgcc
      721 tttttgaaga aactccacga agaggaaatc caggagctgc aggctcagat tcaggaacag
      781 catgtccaaa tcgatgtgga tgtttccaag cctgacctca cggctgccct gcgtgacgta
      841 cgtcagcaat atgaaagtgt ggctgccaag aacctgcagg aggcagaaga atggtacaaa
      901 tccaagtttg ctgacctctc tgaggctgcc aaccggaaca atgacgccct gcgccaggca
      961 aagcaggagt ccactgagta ccggagacag gtgcagtccc tcacctgtga agtggatgcc
     1021 cttaaaggaa ccaatgagtc cctggaacgc cagatgcgtg aaatggaaga gaactttgcc
     1081 gttgaagctg ctaactacca agacactatt ggccgcctgc aggatgagat tcagaatatg
     1141 aaggaggaaa tggctcgtca ccttcgtgaa taccaagacc tgctcaatgt taagatggcc
     1201 cttgacattg agattgccac ctacaggaag ctgctggaag gcgaggagag caggatttct
     1261 ctgcctcttc caaacttttc ctccctgaac ctgagggaaa ctaatctgga ttcactccct
     1321 ctggttgata cccactcaaa aaggacactt ctgattaaga cggttgaaac tagagatgga
     1381 caggttatca acgaaacttc tcagcatcac gatgaccttg aaagcagcgg tggagcaagc
     1441 gcagccagtg gtagcgcgga tccaccggtc gccaccatgg tgagcaaggg cgaggaggtc
     1501 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     1561 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     1621 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     1681 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     1741 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     1801 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     1861 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     1921 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     1981 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     2041 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     2101 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat
     2161 ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg
     2221 gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     2281 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2341 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     2401 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     2461 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     2521 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     2581 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2641 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     2701 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     2761 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     2821 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     2881 tacggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac
     2941 cac
//
