LOCUS       sequence-89279-e        4755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4755)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..1455
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggaggt catcaaagag
       61 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
      121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      181 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
      241 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
      301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
      361 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
      421 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
      481 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
      541 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
      601 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
      661 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc
      721 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc
      781 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
      841 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      901 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
      961 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     1021 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     1081 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     1141 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     1201 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     1261 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     1321 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     1381 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     1441 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgaattc gggaaactcg
     1501 agaacaaagc ttgaaaacgg ccgcatgcca gtgtttcata cgcgcacgat cgagagcatc
     1561 ctggagccgg tggcacagca gatctcccac ctggtgataa tgcacgagga gggcgaggtg
     1621 gacggcaaag ccattcctga cctcaccgcg cccgtggccg ccgtgcaggc ggccgtcagc
     1681 aacctcgtcc gggttggaaa agagactgtt caaaccactg aggatcagat tttgaagaga
     1741 gatatgccac cagcatttat taaggttgag aatgcttgca ccaagcttgt ccaggcagct
     1801 cagatgcttc agtcagaccc ttactcagtg cctgctcgag attatctaat tgatgggtca
     1861 aggggcatcc tctctggaac atcagacctg ctccttacct tcgatgaggc tgaggtccgt
     1921 aaaattatta gagtttgcaa aggaattttg gaatatctta cagtggcaga ggtggtggag
     1981 actatggaag atttggtcac ttacacaaag aatcttgggc caggaatgac taagatggcc
     2041 aagatgattg acgagagaca gcaggagctc actcaccagg agcaccgagt gatgttggtg
     2101 aactcgatga acaccgtgaa agagttgctg ccagttctca tttcagctat gaagattttt
     2161 gtaacaacta aaaactcaaa aaaccaaggc atagaggaag ctttaaaaaa tcgcaatttt
     2221 actctagaaa aaatgagtgc tgaaattaat gagataattc gtgtgttaca actcacctct
     2281 tgggatgaag atgcctgggc cagcaaggac actgaagcca tgaagagagc attggcctcc
     2341 atagactcca aactgaacca ggccaaaggt tggctccgtg accctagtgc ctccccaggg
     2401 gatgctggtg agcaggccat cagacagatc ttagatgaag ctggaaaagt tggtgaactc
     2461 tgtgcaggca aagaacgcag ggagattctg ggaacttgca aaatgctagg gcagatgact
     2521 gatcaagtgg ctgacctccg tgccagagga caaggatcct caccggtggc catgcagaaa
     2581 gctcagcagg tatctcaggg tctggatgtg ctcacagcaa aagtggaaaa tgcagctcgc
     2641 aagctggaag ccatgaccaa ctcaaagcag agcattgcaa agaagatcga tgctgctcag
     2701 aactggcttg cagatccaaa tggtggaccg gaaggagaag agcagattcg aggtgctttg
     2761 gctgaagctc ggaaaatagc agaattatgt gatgatccta aagaaagaga tgacattcta
     2821 cgttcccttg gggaaatatc tgctctgact tctaaattag cagatctacg aagacagggg
     2881 aaaggagatt ctccagaggc tcgagccttg gccaaacagg tggccacggc cctgcagaac
     2941 ctgcagacca aaaccaaccg ggctgtggcc aacagcagac cggccaaagc agctgtacac
     3001 cttgagggca agattgagca agcacagcgg tggattgata atcccacagt ggatgaccgt
     3061 ggagtcggtc aggctgccat ccgggggctt gtggccgaag ggcatcgtct ggctaatgtt
     3121 atgatggggc cttatcggca agatcttctc gccaagtgtg accgagtgga ccagctgaca
     3181 gcccagctgg ctgacctggc tgccagaggg gaaggggaga gtcctcaggc acgagcactt
     3241 gcatctcagc tccaagactc cttaaaggat ctaaaagctc ggatgcagga ggccatgact
     3301 caggaagtgt cagatgtttt cagcgatacc acaactccca tcaagctgtt ggcagtggca
     3361 gccacggcgc ctcctgatgc gcctaacagg gaagaggtat ttgatgagag ggcagctaac
     3421 tttgaaaacc attcaggaaa gcttggtgct acggccgaga aggcggctgc ggttggtact
     3481 gctaataaat caacagtgga aggcattcag gcctcagtga agacggcccg agaactcaca
     3541 ccccaggtgg tctcggctgc tcgtatctta cttaggaacc ctggaaatca agctgcttat
     3601 gaacattttg agaccatgaa gaaccagtgg atcgataatg ttgaaaaaat gacagggctg
     3661 gtggacgaag ccattgatac caaatctctg ttggatgctt cagaagaagc aattaaaaaa
     3721 gacctggaca agtgcaaggt agctatggcc aacattcagc ctcagatgct ggttgctggg
     3781 gcaaccagta ttgctcgtcg ggccaaccgg atcctgctgg tggctaagag ggaggtggag
     3841 aattccgagg atcccaagtt ccgtgaggct gtgaaagctg cctctgatga attgagcaaa
     3901 accatctccc cgatggtgat ggatgcaaaa gctgtggctg gaaacatttc cgaccctgga
     3961 ctgcaaaaga gcttcctgga ctcaggatat cggatcctgg gagctgtggc caaggtcaga
     4021 gaagccttcc aacctcagga gcctgacttc ccgccgcctc caccagacct tgaacaactc
     4081 cgactaacag atgagcttgc tcctcccaaa ccacctctgc ctgaaggtga ggtccctcca
     4141 cctaggcctc caccaccaga ggaaaaggat gaagagttcc ctgagcagaa ggccggggag
     4201 gtgattaacc agccaatgat gatggctgcc agacagctcc atgatgaagc tcgcaaatgg
     4261 tccagcaagg gcaatgacat cattgcagca gccaagcgca tggctctgct gatggctgag
     4321 atgtctcggc tggtaagagg gggcagtggt accaagcggg cactcattca gtgtgccaag
     4381 gacatcgcca aggcctcaga tgaggtgact cggttggcca aggaggttgc caagcagtgc
     4441 acagataaac ggattagaac caacctctta caggtatgtg agcgaatccc aaccataagc
     4501 acccagctca aaatcctgtc cacagtgaag gccaccatgc tgggccggac caacatcagt
     4561 gatgaggagt ctgagcaggc cacagagatg ctggttcaca atgcccagaa cctcatgcag
     4621 tctgtgaagg agactgtgcg ggaagctgaa gctgcttcaa tcaaaattcg aacagatgct
     4681 ggatttacac tgcgctgggt tagaaagact ccctggtacc agtaggcggc cgcgactcta
     4741 gatcataatc agcca
//
