LOCUS       sequence-89281-e        6745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6745)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..1455
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     RBS             3156..3164
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggaggt catcaaagag
       61 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
      121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      181 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
      241 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
      301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
      361 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
      421 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
      481 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
      541 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
      601 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
      661 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc
      721 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc
      781 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
      841 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      901 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
      961 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     1021 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     1081 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     1141 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     1201 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     1261 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     1321 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     1381 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     1441 gacgagctgt acaagtccgg aagtgctggg agcgcaagcg gaagcggagg ctccggtgag
     1501 gaaacagcta tatgggaaca acatacagtg acgcttcaca gggctcctgg atttggattt
     1561 ggaattgcaa tatctggtgg acgagataat cctcattttc agagtgggga aacgtcaata
     1621 gtgatttcag atgtgctgaa aggaggacca gctgaaggac agctacagga aaatgaccga
     1681 gttgcaatgg ttaacggagt ttcaatggat aatgttgaac atgcttttgc tgttcagcaa
     1741 ctaaggaaaa gtgggaaaaa tgcaaaaatt acaattagaa ggaagaagaa agttcaaata
     1801 ccagtaagtc gtcctgatcc tgaaccagta tctgataatg aagaagatag ttatgatgag
     1861 gaaatacatg atccaagaag tggccggagt ggtgtggtta acagaaggag tgagaagatt
     1921 tggccgaggg atagaagtgc aagtagagag aggagcttgt ccccgcggtc agacaggcgg
     1981 tcagtggctt ccagccagcc tgctaaacct actaaagtca cactggtgaa atcccggaaa
     2041 aatgaagaat atggtcttcg attggcaagc catatatttg ttaaggaaat ttcacaagat
     2101 agtttggcag caagagatgg caatattcaa gaaggtgatg ttgtattgaa gataaatggt
     2161 actgtgacag aaaatatgtc attgacagat gcaaagacat tgatagaaag gtctaaaggc
     2221 aaattaaaaa tggtagttca aagagatgaa cgggctacgc tattgaatgt ccctgatctt
     2281 tctgacagca tccactctgc taatgcctct gagagagacg acatttcaga aattcagtca
     2341 ctggcatcag atcattctgg tcgatcacac gataggcctc cccgccgcag ccggtcacga
     2401 tctcctgacc agcggtcaga gccttctgat cattccaggc actcgccgca gcagccaagc
     2461 aatggcagtc tgcggagtag agatgaagag agaatttcta aacctggggc tgtctcaact
     2521 cctgtaaagc atgctgatga tcacacacct aaaacagtgg aagaagttac agttgaaaga
     2581 aatgagaaac aaacaccttc tcttccagaa ccaaagcctg tgtatgccca agttggcaac
     2641 cagatgtgga tttacctgtc agtccatctg atggtgtcct acctaattca actcatgaag
     2701 atgggatttc ttcggcccag catgaaattg gtaaaattca gaaaaggaga tagtgtgggt
     2761 ttgcggctgg ctggtggaaa tgatgttgga atatttgtag ctggcgttct agaagatagc
     2821 cctgcagcca aggaaggctt agaggaaggt gatcaaattc tcagggtaaa caacgtagat
     2881 tttacaaata tcataagaga agaagccgtc cttttcctgc ttgacctccc taaaggagaa
     2941 gaagtgacca tattggctca gaagaagaag gatgtttatc gtcgcattgt agaatcagat
     3001 gtaggagatt ctttctatat tagaacccat tttgaatatg aaaaggaatc tccctatgga
     3061 cttagtttta acaaaggaga ggtgttccgt gctgtggata ccttgtacaa tggaaaactg
     3121 ggctcttggc ttgctattcg aattggtaaa aatcataagg aggtagaacg aggcatcatc
     3181 cctaataaga acagagctga gcagctcgcc agtgtacagt atacacttcc aaaaacagca
     3241 ggcggagacc gtgctgactt ctggagattc agaggtcttc gcagctccaa gagaaatctt
     3301 cgaaaaagca gagaggattt gtccgctcag cctgttcaaa caaagtttcc agcttatgaa
     3361 agagtggttc ttcgagaagc tggatttctg aggcctgtaa ccatttttgg accaatagct
     3421 gatgttgcca gagaaaagct ggcaagagaa gaaccagata tttatcaaat tgcaaagagt
     3481 gaaccacgag acgctggaac tgaccaacgt agctctggct atattcgcct gcatacaata
     3541 aagcaaatca tagatcaaga caaacatgct ttattagatg taacaccaaa tgcagttgat
     3601 cgtcttaact atgcccagtg gtatccaatt gttgtatttc ttaaccctga ttctaagcaa
     3661 ggagtaaaaa caatgagaat gaggttatgt ccagaatctc ggaaaagtgc caggaagtta
     3721 tacgagcgat ctcataaact tgctaaaaat aatcaccatc tttttacaac tacaattaac
     3781 ttaaattcaa tgaatgatgg ttggtatggt gcgctgaaag aagcagttca acaacagcaa
     3841 aaccagctgg tatgggtttc cgagggaaag gcggatggtg ctacaagtga tgaccttgat
     3901 ttgcatgatg atcgtctgtc ctacctgtca gctccaggta gtgaatactc aatgtatagc
     3961 acggacagta gacacacttc tgactatgaa gacacagaca cagaaggcgg ggcctacact
     4021 gatcaagaac tagatgaaac tcttaatgat gaggttggga ctccaccgga gtctgccatt
     4081 acacggtcct ctgagcctgt aagagaggac tcctctggaa tgcatcatga aaaccaaaca
     4141 tatcctcctt actcaccaca agcgcagcca caaccaattc atagaataga ctcccctgga
     4201 tttaagccag cctctcaaca gaaagcagaa gcttcatctc cagtccctta cctttcgcct
     4261 gaaacaaacc cagcatcatc aacctctgct gttaatcata atgtaaattt aactaatgtc
     4321 agactggagg agcccacccc agctccttcc acctcttact caccacaagc tgattcttta
     4381 agaacaccaa gtactgaggc agctcacata atgctaagag atcaagaacc atcattgtcg
     4441 tcgcatgtag atccaacaaa ggtgtataga aaggacccat atcccgagga aatgatgagg
     4501 cagaaccatg ttttgaaaca gccagccgtt agtcacccag ggcacaggcc agacaaagag
     4561 cctaatctga cctatgaacc ccaactccca tacgtagaga aacaagccag cagagacctg
     4621 gagcagccca catacagata cgagtcctca agctatacgg accagttttc tcgaaactat
     4681 gaacatcgtc tgcgatacga agatcgcgtc cccatgtatg aagaacagtg gtcatattat
     4741 gatgacaaac agccctaccc atctcggcca ccttttgata atcagcactc tcaagacctt
     4801 gactccagac agcatcccga agagtcctca gaacgagggt actttccacg ttttgaagag
     4861 ccagcccctc tgtcttacga cagcagacca cgttacgaac aggcacctag agcatccgcc
     4921 ctgcggcacg aagagcagcc agctcctggg tatgacacac atggtagact cagaccggaa
     4981 gcccagcccc acccttcagc agggcccaag cctgcagagt ccaagcagta ttttgagcaa
     5041 tattcacgca gttacgagca agtaccaccc caaggattta cctctagagc aggtcatttt
     5101 gagcctctcc atggtgctgc agctgtccct ccgctgatac cttcatctca gcataagcca
     5161 gaagctctgc cttcaaacac caaaccactg cctccacccc caactcaaac cgaagaagag
     5221 gaagatccag caatgaagcc acagtctgta ctcaccagag ttaagatgtt tgaaaacaaa
     5281 aggtctgcat ccttagagac caagaaggat gtaaatgaca ctggcagttt taagcctcca
     5341 gaagtagcat ctaaaccttc aggtgctccc atcattggtc ccaaacccac ttctcagaat
     5401 caattcagtg aacatgacaa aactctgtac aggattccag aacctcaaaa acctcaactg
     5461 aagccacctg aagatattgt tcggtccaat cattatgacc ctgaagaaga tgaagaatat
     5521 tatcgaaaac agctgtcata ctttgaccga agaagttttg agaataagcc tcctgcacac
     5581 attgctgcca gccatctctc cgagcctgca aagccagctc attctcagaa tcaatcaaat
     5641 ttttctagtt attcttcaaa gggaaagcct cctgaagctg atggtgtgga tagatcattt
     5701 ggcgagaaac gctatgaacc catccaggcc actccccctc ctcctccatt gccctcgcag
     5761 tatgcccagc catctcagcc tgtcaccagc gcgtctctcc acatacattc taagggagca
     5821 catggtgaag gtaattcagt gtcattggat tttcagaatt ccttagtgtc caaaccagac
     5881 ccacctccat ctcagaataa gccagcaact ttcagaccac caaaccgaga agatactgct
     5941 caggcagctt tctatcccca gaaaagtttt ccagataaag ccccagttaa tggaactgaa
     6001 cagactcaga aaacagtcac tccagcatac aatcgattca caccaaaacc atatacaagt
     6061 tctgcccgac catttgaacg caagtttgaa agtcctaaat tcaatcacaa tcttctgcca
     6121 agtgaaactg cacataaacc tgacttgtct tcaaaaactc ccacttctcc aaaaactctt
     6181 gtgaaatcgc acagtttggc acagcctcct gagtttgaca gtggagttga aactttctct
     6241 atccatgcag agaagcctaa atatcaaata aataatatca gcacagtgcc taaagctatt
     6301 cctgtgagtc cttcagctgt ggaagaggat gaagatgaag atggtcatac tgtggtggcc
     6361 acagcccgag gcatatttaa cagcaatggg ggcgtgctga gttccataga aactggtgtt
     6421 agtataatta tccctcaagg agccattccc gaaggagttg agcaggaaat ctatttcaag
     6481 gtctgccggg acaacagcat ccttccacct ttagataaag agaaaggtga aacactgctg
     6541 agtcctttgg tgatgtgtgg tccccatggc ctcaagttcc tgaagcctgt ggagctgcgc
     6601 ttaccacact gtgatcctaa aacctggcaa aacaagtgtc ttcccggaga tccaaattat
     6661 ctcgttggag caaactgtgt ttctgtcctt attgaccact tttaaggatc caccggatct
     6721 agataactga tcataatcag ccata
//
