LOCUS       sequence-89282-e        3263 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Shaner NC, Patterson GH, Davidson MW
  TITLE     Advances in fluorescent protein technology
  JOURNAL   J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60
  PUBMED    18057027
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-12-15"; volume: "120(Pt 24)"; pages: "4247-6"
COMMENT     Sequence Label: tdTomato-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..3263
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1786..1795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1792..3222
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccatg
       61 gcggcccccc gcccgtctcc cgcgatcccc gtttcggtct cggctccggc tttttacgcc
      121 ccgcagaaga agttcggccc tgtggtggcc ccaaagccca aagtgaatcc cttccggccc
      181 agggacagcg agcctccccc ggcacccggg gcccagcgcg cacagatggg ccgggtgggc
      241 gatattcccc cgccgccccc ggaagacttt cccctgcctc cacctcccct tgctggggat
      301 ggcgacgatg cagagggtgc tctgggaggt gccttcccgc cgccccctcc cccgatcgag
      361 gaatcatttc cccctgcgcc tctggaggag gagatcttcc cttccccgcc gcctcctccg
      421 gaggaggagg gagggcctga ggcccccata ccgcccccac cacagcccag ggagaaggtg
      481 agcagtattg atttggagat cgactctctg tcctcactgc tggatgacat gaccaagaat
      541 gatcctttca aagcccgggt gtcatctgga tatgtgcccc caccagtggc cactccattc
      601 agttccaagt ccagtaccaa gcctgcagcc gggggcacag cacccctgcc tccttggaag
      661 tccccttcca gctcccagcc tctgccccag gttccggctc cggctcagag ccagacacag
      721 ttccatgttc agccccagcc ccagcccaag cctcaggtcc aactccatgt ccagtcccag
      781 acccagcctg tgtctttggc taacacccag ccccgagggc ccccagcctc atctccggct
      841 ccagccccta agttttctcc agtgactcct aagtttactc ctgtggcttc caagttcagt
      901 cctggagccc caggtggatc tgggtcacaa ccaaatcaaa aattggggca ccccgaagct
      961 ctttctgctg gcacaggctc ccctcaacct cccagcttca cctatgccca gcagagggag
     1021 aagccccgag tgcaggagaa gcagcacccc gtgcccccac cggctcagaa ccaaaaccag
     1081 gtgcgctccc ctggggcccc agggcccctg actctgaagg aggtggagga gctggagcag
     1141 ctgacccagc agctaatgca ggacatggag catcctcaga ggcagaatgt ggctgtcaac
     1201 gaactctgcg gccgatgcca tcaacccctg gcccgggcgc agccagccgt ccgcgctcta
     1261 gggcagctgt tccacatcgc ctgcttcacc tgccaccagt gtgcgcagca gctccagggc
     1321 cagcagttct acagtctgga gggggcgccg tactgcgagg gctgttacac tgacaccctg
     1381 gagaagtgta acacctgcgg ggagcccatc actgaccgca tgctgagggc cacgggcaag
     1441 gcctatcacc cgcactgctt cacctgtgtg gtctgcgccc gccccctgga gggcacctcc
     1501 ttcatcgtgg accaggccaa ccggccccac tgtgtccccg actaccacaa gcagtacgcc
     1561 ccgaggtgct ccgtctgctc tgagcccatc atgcctgagc ctggccgaga tgagactgtg
     1621 cgagtggtcg ccctggacaa gaacttccac atgaagtgtt acaagtgtga ggactgcggg
     1681 aagcccctgt cgattgaggc agatgacaat ggctgcttcc ccctggacgg tcacgtgctc
     1741 tgtcggaagt gccacactgc tagagcccag acggatccac cggtcgccac catggtgagc
     1801 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     1861 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1921 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     1981 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     2041 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2101 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     2161 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     2221 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     2281 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     2341 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     2401 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     2461 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc
     2521 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     2581 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2641 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     2701 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     2761 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2821 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     2881 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     2941 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     3001 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     3061 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     3121 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     3181 caccacctgt tcctgtacgg catggacgag ctgtacaagt aagcggccgc gactctagat
     3241 cataatcagc cataccacat gag
//
