LOCUS       sequence-89286-e        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mTurquoise-mClover-FRET-10
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      452..461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(458..460,461..463,464..1177)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(503..524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(764..783)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 caagttcggc attggcgaag gttacgtcca gagcacatct cagcgacgta cctacaagac
       61 ccgccgaggt gaagttcgag gggcgacacc tggtgatgca tcgagctgag ggcatcgact
      121 cagagacgcc accatctggg cacagctgag tacactcaca gcacacgtct tatatcacgc
      181 gacagcagag acggcatcaa ggctaacttc aagatccgcc acaacgtgag gacggcagcg
      241 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      301 ccgacaaacc actactgagc catcagtcca agctgagcaa agaccccaac gagaagcgcg
      361 atcacatggt ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      421 gtacaagtcc ggactcagat ccccaccggt cgccaccatg gtgagcaagg gcgaggagct
      481 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      541 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      601 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctgggg
      661 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      721 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      781 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      841 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
      901 cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      961 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     1021 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1081 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1141 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata
     1201 atcagccata ccacattgta gagaagccc
//
