LOCUS       sequence-89287-e        1270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mPAGFP-mRuby2-FRET-10
FEATURES             Location/Qualifiers
     source          1..1270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..746
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      771..780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(822..843)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accattgtct agtgacgtca gatccgctag ccaccatggt gtctaagggc gaagagctga
       61 tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc ggtcaacggc caccaattca
      121 aatgcacagg tgaaggagaa ggcaatccgt acatgggaac tcaaaccatg aggatcaaag
      181 tcatcgaggg aggacccctg ccatttgcct ttgacattct tgccacgtcg ttcatgtatg
      241 gcagccgtac ttttatcaag tacccgaaag gcattcctga tttctttaaa cagtcctttc
      301 ctgagggttt tacttgggaa agagttacga gatacgaaga tggtggagtc gtcaccgtca
      361 tgcaggacac cagccttgag gatggctgtc tcgtttacca cgtccaagtc agaggggtaa
      421 actttccctc caatggtccc gtgatgcaga agaagaccaa gggttgggag cctaatacag
      481 agatgatgta tccagcagat ggtggtctga ggggatacac tcatatggca ctgaaagttg
      541 atggtggtgg ccatctgtct tgctctttcg taacaactta caggtcaaaa aagaccgtcg
      601 ggaacatcaa gatgcccggt atccatgccg ttgatcaccg cctggaaagg ttagaggaaa
      661 gtgacaatga aatgttcgta gtacaacgcg aacacgcagt tgccaagttc gccgggcttg
      721 gtggtgggat ggacgagctg tacaagtccg gactcagatc cccaccggtc gccaccatgg
      781 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      841 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      901 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      961 tgaccacctt cagctacgcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1021 cgacttcttc aagtccgcca tgcccgaggc tacgtccagg agcgcacatc ttcttcagga
     1081 cgacggcact acagacccgc gccgaggtga agtcgagggc gacccctgtg acgcatcgag
     1141 ctgatcatcg actcagaaga cgcacatctg gggccagcct ggatacaact acacagccac
     1201 acgtctatca tggcgacagc taagacgcat cagtcacctc aggatcgctc tcaacatcca
     1261 gggacgtgca
//
