LOCUS       sequence-89288-e        1287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Davidson Lab
  TITLE     FRET Indicators - Live and Fixed Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mPAGFP-mRuby2-FRET-10
FEATURES             Location/Qualifiers
     source          1..1287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      510..519
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(516..518,519..521,522..1232)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(561..582)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(822..841)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1169..1190
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggtatcccgc agaagggcac acttcctgga gttcttaaac agtctttctg aggcttacct
       61 gcaaagctta ccgagatacg agatgtgatc gtcacgtcat gcaaggaaca cccagcctga
      121 gggattgctg tctcgtttac cacgtcaagt cagaggggta aactttcctc caatggtccg
      181 tggatgcaga gagaccaagg gtgggagcct aatacagaag atgatgtatc cagcagatgg
      241 tggtctgagg ggatacactc atatggcact gaaagttgat ggtggtggcc catctgtctt
      301 gctctttcgt aacaacttac aggtcaaaaa agaccgtcgg gaacatcaag atgcccggta
      361 tccatgccgt tgatcaccgc ctggaaaggt tagaggaaag tgacaatgaa atgttcgtag
      421 tacaacgcga acacgcagtt gccaagttcg ccgggcttgg tggtgggatg gacgagctgt
      481 acaagtccgg actcagatcc ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
      541 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      601 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      661 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc agctacggcg
      721 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      781 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      841 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      901 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      961 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     1021 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1081 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagccaccag tccaagctga
     1141 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1201 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat
     1261 cagccatacc acattgtaga gtttcct
//
