LOCUS       sequence-89295-e        1280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1280)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ALK1-C-18
FEATURES             Location/Qualifiers
     source          1..1280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..591
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 ccaatgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatgga caaagactgc
       61 gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa
      121 cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa
      181 gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg
      241 ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac
      301 cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg
      361 gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg caatcccgcc
      421 gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct gatcccctgc
      481 caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct cgccgtgaaa
      541 gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg ttccggactc
      601 agatctcgag ctcaagcttc gaattcaggg tctggaagtg gtagcacctt gggctccccc
      661 aggaaaggcc ttctgatgct gctgatggcc ttggtgaccc agggagaccc tgtgaagccg
      721 tctcggggcc cgctggtgac ctgcacgtgt gagagcccac attgcaaggg gcctacctgc
      781 cggggggcct ggtgcacagt agtgctggtg cgggaggagg ggaggcaccc ccaggaacat
      841 cggggctgcg ggaacttgca cagggagctc tgcagggggc gccccaccga gttcgtcaac
      901 cactactgct gcgacagcca cctctgcaac cacaacgtgt ccctggtgct ggaggccacc
      961 caacctcctt cggagcagcc gggaacagat ggccagctgg gccctgatcc tgggcccgtg
     1021 ctggccttgc tggccctggt ggccctgggt gtcctggcct gtggcatgtc cgacggaggc
     1081 aggagaagca gcgtgctgca cagcgagctg ggagagtcca gtctcatctg aaagcatctg
     1141 agcaggcgac agcatgtggg ggacctcctg gacagtgact gcacccacaa gggagtgctc
     1201 agctccccct cctgggtaca gtctggactg caagttgtct tggtgatgtg tgtggaaagc
     1261 gccctatggg ccaatgtgtg
//
