LOCUS       sequence-89299-e        1330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1330)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..591
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 accatgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatgga caaagactgc
       61 gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa
      121 cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa
      181 gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg
      241 ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac
      301 cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg
      361 gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg caatcccgcc
      421 gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct gatcccctgc
      481 caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct cgccgtgaaa
      541 gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg ttccaaactc
      601 agatctcgag ctcaagcttc gaattctgca gtcgacatgg ctgatgactt tggcttcttc
      661 tcgtcgtcgg agagtggtgc cccggaggcg gcggaggagg acccggcggc cgccttcctg
      721 gcccagcagg agagcgagat tgcaggcata gagaacgacg agggcttcgg ggcacctgcc
      781 ggcagccatg cggcccccgc acagccgggc cccacgagtg gggctggttc tgaggacatg
      841 gggaccacag tcaatggaga tgtgtttcag gaggccaacg gtcctgctga tggctacgca
      901 gccattgccc aggctgacag gctgacccag gagcctgaga gcatccgcaa gtggcgagag
      961 gagcagagga aacggctgca agagctggat gctgcatcta aggtcacgga acaggaatgg
     1021 cgggagaagg ccaagaagga cctggaggag tggaaccagc gccagagtga acaagtagag
     1081 aagaacaaga tcaacaaccg ggcatccgag gaggctttcg tgaaggaatc caaggaggag
     1141 accccaggca cagagtggga gaaggtggcc cagctatgtg acttcaaccc caagagcagc
     1201 aagcagtgca aagatgtgtc ccgcctgcgc tcggtgctca tgtccctgaa gcagacgcca
     1261 ctgtcccgct agggatccac cggatctaga taactgatca taatcagcca taccacattg
     1321 tagagtagtc
//
