LOCUS       sequence-89303-e        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: 
            N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Integrin-Alpha2-N-18
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      524..533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             530..1075
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 cgtatacgca tctgaatcag aagtcaatct atggtcatcg tcagctggac tcagttatgc
       61 gaattcatca tgactagaca acttctcgat ctcaaaggga agttcagcca gcagatgact
      121 gcagactgct ccgtagtatg tactgctggt gagacgtcac atgatgagat accttgtatg
      181 tgactacaga tttgacggga ctttgcatca tcacgtcaga cagtacagct acgcagctgc
      241 agaatcacac tatactgaga tatatgtgat gagatacact gttacgatcc cctgatgata
      301 tgaaactgat gagaaagcga agtaccacag gagtataata ggaagtataa ttgctggaat
      361 ccttttgctg ttagctctgg ttgcaatttt atggaagctc ggcttcttca aaagaaaata
      421 tgaaaagatg accaaaaatc cagatgagat tgatgagacc acagagctca gtagcggcag
      481 cgcaggcgga agcggggtac cgcgggcccg ggatccaccg gtcgccacca tggacaaaga
      541 ctgcgaaatg aagcgcacca ccctggatag ccctctgggc aagctggaac tgtctgggtg
      601 cgaacagggc ctgcaccgta tcatcttcct gggcaaagga acatctgccg ccgacgccgt
      661 ggaagtgcct gccccagccg ccgtgctggg cggaccagag ccactgatgc aggccaccgc
      721 ctggctcaac gcctactttc accagcctga ggccatcgag gagttccctg tgccagccct
      781 gcaccaccca gtgttccagc aggagagctt tacccgccag gtgctgtgga aactgctgaa
      841 agtggtgaag ttcggagagg tcatcagcta cagccacctg gccgccctgg ccggcaatcc
      901 cgccgccacc gccgccgtga aaaccgccct gagcggaaat cccgtgccca ttctgatccc
      961 ctgccaccgg gtggtgcagg gcgacctgga cgtggggggc tacgagggcg ggctcgccgt
     1021 gaaagagtgg ctgctggccc acgagggcca cagactgggc aagcctgggc tgggttaagc
     1081 ggccgcgact ctagatcata atcagccata ccacatttgt agagtagcct
//
