LOCUS       sequence-89309-e        1256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1256)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      650..659
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             656..1201
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 atttgtctct aagcctgtag atattatcga agatgaggtc gctgaactat gcagtgtgct
       61 gcttaacaag cagaaatttc gaagctacaa ccatgcagct tgtggactac tgtattttca
      121 tctgtgagga ataatcgcta tgatgggacg tctgttgaga gagcggcagc agcatgatac
      181 tacaagagga ggcgatttca tcctgggctg agaggtggat ggaatggatt atcctgtgcg
      241 tccgagaggc aacaaggttt gctgctgcct attgtagatc tgggaagggg cccatctgat
      301 ggagctgcag acttaccgtt accacggaca cagtatgagt gaccctggag tcagttaccg
      361 tacacgagaa gaaattcagg aagtaagaag taagagtgac cctattatgc ttctcaagga
      421 caggatggtg aacagcaatc ttgccagtgt ggaagaacta aaggaaattg atgtggaagt
      481 gaggaaggag attgaggatg ctgcccagtt tgccacggcc gatcctgagc cacctttgga
      541 agagctgggc taccacatct actccagcga cccacctttt gaagttcgtg gtgccaatca
      601 gtggatcaag tttaagtcag tcagtagctc cggaggggat ccaccggtcg ccaccatgga
      661 caaagactgc gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc
      721 tgggtgcgaa cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga
      781 cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc
      841 caccgcctgg ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc
      901 agccctgcac cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact
      961 gctgaaagtg gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg
     1021 caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct
     1081 gatcccctgc caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct
     1141 cgccgtgaaa gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg
     1201 ttaagcggcc gcgactctag atcataatca gccataccac attgtagaag ttgcct
//
