LOCUS       sequence-89311-e        1267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1267)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..1267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      663..672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             669..1214
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 acaacttctc tctgggcggg ggatgtctac ctccacagta tcacctctat tggaagctag
       61 gtcattcggc acagactcca aggctccaat gcctgtaaag aacgactact gctgctgttg
      121 actgggcttt cctgcgttct gacctactcg ttctgtggat gacctgctac agtgggcagg
      181 tggactgcag atacatgctc cagaagtcct agaatcagat gatttgagat gtgagtctca
      241 agcagaccga tgtctactcc atggctctgg tgctctggga aatgacatct cgctgtaatg
      301 cagtgggaga agtaaaagat tatgagcctc catttggttc caaggtgcgg gagcacccct
      361 gtgtcgaaag catgaaggac aacgtgttga gagatcgagg gcgaccagaa attcccagct
      421 tctggctcaa ccaccagggc atccagatgg tgtgtgagac gttgactgag tgctgggacc
      481 acgacccaga ggcccgtctc acagcccagt gtgtggcaga acgcttcagt gagctggagc
      541 atctggacag gctctcgggg aggagctgct cggaggagaa gattcctgaa gacggctccc
      601 taaacactac caaaagcagc ggtggagcaa gcgcagccag tggtagcgcg gatccaccgg
      661 tcgccaccat ggacaaagac tgcgaaatga agcgcaccac cctggatagc cctctgggca
      721 agctggaact gtctgggtgc gaacagggcc tgcaccgtat catcttcctg ggcaaaggaa
      781 catctgccgc cgacgccgtg gaagtgcctg ccccagccgc cgtgctgggc ggaccagagc
      841 cactgatgca ggccaccgcc tggctcaacg cctactttca ccagcctgag gccatcgagg
      901 agttccctgt gccagccctg caccacccag tgttccagca ggagagcttt acccgccagg
      961 tgctgtggaa actgctgaaa gtggtgaagt tcggagaggt catcagctac agccacctgg
     1021 ccgccctggc cggcaatccc gccgccaccg ccgccgtgaa aaccgccctg agcggaaatc
     1081 ccgtgcccat tctgatcccc tgccaccggg tggtgcaggg cgacctggac gtggggggct
     1141 acgagggcgg gctcgccgtg aaagagtggc tgctggccca cgagggccac agactgggca
     1201 agcctgggct gggttaagcg gccgcgactc tagatcataa tcagccatac cacatgtaga
     1261 gaacccc
//
