LOCUS       sequence-89312-e        1291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1291)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..1291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..590
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 acatgctagt gacgtcagat ccgctagcgc taccggtcgc caccatggac aaagactgcg
       61 aaatgaagcg caccaccctg gatagccctc tgggcaagct ggaactgtct gggtgcgaac
      121 agggcctgca ccgtatcatc ttcctgggca aaggaacatc tgccgccgac gccgtggaag
      181 tgcctgcccc agccgccgtg ctgggcggac cagagccact gatgcaggcc accgcctggc
      241 tcaacgccta ctttcaccag cctgaggcca tcgaggagtt ccctgtgcca gccctgcacc
      301 acccagtgtt ccagcaggag agctttaccc gccaggtgct gtggaaactg ctgaaagtgg
      361 tgaagttcgg agaggtcatc agctacagcc acctggccgc cctggccggc aatcccgccg
      421 ccaccgccgc cgtgaaaacc gccctgagcg gaaatcccgt gcccattctg atcccctgcc
      481 accgggtggt gcagggcgac ctggacgtgg ggggctacga gggcgggctc gccgtgaaag
      541 agtggctgct ggcccacgag ggccacagac tgggcaagcc tgggctgggt tccggactca
      601 gatctggcag cggtggaggc agcgcatccg gcggaagcgg aagcgtgcgt gagtgcatct
      661 ccatccacgt tggccaggct ggtgtccaga ttggcaatgc ctgctgggag ctctactgcc
      721 tggaacacgg catccagccc gatggccaga tgccaagtga caagaccatt gggggaggag
      781 atgactcctt caacaccttc ttcagtgaga cgggcgctgg caagcacgtg ccccgggctg
      841 tgtttgtaga cttggaaccc acagtcattg atgaagttcg cactggcacc taccgccagc
      901 tcttccaccc tgagcagctc atcacagcaa gagatgctgc catactatgc ccgaggcact
      961 acaccattgg caggagatca ttgacttgtg ttggaccgat tcgcagctgg ctgacagtgc
     1021 accggtcttc aggcttcttg gtttccacag ctttggtggg ggactggttc tgggttcacc
     1081 tccctgctca tggaacgtct tctcagtgat tatggcagaa gtcaagcctg ggatttccat
     1141 taccaggacc caggttccca gctgtgattg acctaattca ttctcaacaa actggaaact
     1201 ctatatggct ctgtgacaat agccttgaac tgctgataac ctgactagcc accacttaag
     1261 tctatgccga tggttcctct acacggtctc g
//
