LOCUS       sequence-89315-e        1228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: N/A, Emission: N/A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1228)
  TITLE     Davidson plasmids
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Vinculin-23
FEATURES             Location/Qualifiers
     source          1..1228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      623..632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             629..1174
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 agtttctttc acttaaggct ttacacagag ggaagatgga gagttcctga gcgaagccgg
       61 gaggtgatac aggcaatgat gatgctgcag acagctcatg atgaggctgc aatgtcagca
      121 ggcatgacat catgcagcag cagcgcatgc tctgctgatg ctgagatgtc tcggctggta
      181 agaggggcag tggtaccaag cgggcactca ttcagtgtgc caaggacatc gccaaggctc
      241 agatgaggtg actcggttgg ccaaggaggt tgccaagcag tgcacagata aacggattag
      301 aaccaacctc ttacaggtat gtgagcgaat cccaaccata agcacccagc tcaaaatcct
      361 gtccacagtg aaggccacca tgctgggccg gaccaacatc agtgatgagg agtctgagca
      421 ggccacagag atgctggttc acaatgccca gaacctcatg cagtctgtga aggagactgt
      481 gcgggaagct gaagctgctt caatcaaaat tcgaacagat gctggattta cactgcgctg
      541 ggttagaaag actccctggt accagtccgg tggcagcgga attctgcagt cgacggtacc
      601 gcgggcccgg gatccaccgg tcgccaccat ggacaaagac tgcgaaatga agcgcaccac
      661 cctggatagc cctctgggca agctggaact gtctgggtgc gaacagggcc tgcaccgtat
      721 catcttcctg ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg ccccagccgc
      781 cgtgctgggc ggaccagagc cactgatgca ggccaccgcc tggctcaacg cctactttca
      841 ccagcctgag gccatcgagg agttccctgt gccagccctg caccacccag tgttccagca
      901 ggagagcttt acccgccagg tgctgtggaa actgctgaaa gtggtgaagt tcggagaggt
      961 catcagctac agccacctgg ccgccctggc cggcaatccc gccgccaccg ccgccgtgaa
     1021 aaccgccctg agcggaaatc ccgtgcccat tctgatcccc tgccaccggg tggtgcaggg
     1081 cgacctggac gtggggggct acgagggcgg gctcgccgtg aaagagtggc tgctggccca
     1141 cgagggccac agactgggca agcctgggct gggttaagcg gccgcgactc tagatcataa
     1201 tcagccatac cacattgtag agaagccc
//
