LOCUS       sequence-89316-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/Endoplasmic Reticulum, Excitation: 487, 
            Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-VSVG-16
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    138..259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(175..194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     229..248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(266..721)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(403..424)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     615..634
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
       61 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      121 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      181 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      241 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
      301 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      361 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
      421 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      481 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      541 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      601 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      661 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      721 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      781 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      841 aa
//
