LOCUS       sequence-8933-ex        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J
  TITLE     TGFbeta primes breast tumors for lung metastasis seeding through 
            angiopoietin-like 4.
  JOURNAL   Cell. 2008 Apr 4. 133(1):66-77.
  PUBMED    18394990
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Apr 4. 133(1):66-77."
COMMENT     Sequence Label: pRetroSuper shSmad4J puro
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(57..271)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(117..136)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        282..781
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(317..340)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     714..733
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(806..825)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tangggtgag ttggaatgta tctcttgaat acattccaac tgcacaccgg ggatctgtgg
       61 tctcatacag aacttataag attcccaaat ccaaagacat ttcacgttta tggtgatttc
      121 ccagaacaca tagcgacatg caaatattgc agggcgccac tcccctgtcc ctcacagcca
      181 tcttcctgcc agggcgcacg cgcgctgggt gttcccgcct agtgacactg ggcccgcgat
      241 tccttggagc gggttgatga cgtcagcgtt cgaattctac cgggtagggg aggcgctttt
      301 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
      361 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
      421 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
      481 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
      541 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
      601 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
      661 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
      721 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
      781 gacctgcagc ccaagctagc ttaccatgac cgagtacaag cccacggtgc gcctcgcnac
      841 ccgcgangac gtccccaggg ccgtacgcac cctcgccgcc gngttnncga ntaccccgcc
      901 acgggcnaaa cgtngatccg gancgccaca tcgagcgggg tcaccgagct gcanaantnt
      961 tctnncgcgg gtggggnnga natcggaang tgtgggtcgc gnanaaggcc cncggtggcg
     1021 gtntgaacac nccgnaaacg tcaaacgggg cggtnt
//
