LOCUS       sequence-89345-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H4-23
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    36..88
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(436..639)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(465..485)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        640..943
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
       61 actgcagaat tcgaagcttg agctcgagat ccccaccgaa accgtagagg gtgcggccct
      121 ggcgcttgag cgcgtagacc acatccatgg cggtgaccgt cttgcgcttg gcgtgctctg
      181 tataggtcac ggcgtcccgg atcacgttct ccaggaacac cttcagcacc ccgcgagtct
      241 cctcgtagat gaggccggag atgcgcttca cgccgccgcg gcgagcaagg cgccggatgg
      301 ccggcttggt gatgccctgg atattgtcgc gcagtacttt acggtggcgc ttagcgccgc
      361 ctttgccaag acccttcccg cctttgccgc ggccagacat ggtggcggct agcggatctg
      421 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
      481 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
      541 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
      601 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
      661 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
      721 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
      781 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
      841 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
      901 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
      961 gctatgacta atgaccccgt aattgattac tatta
//
