LOCUS       sequence-89350-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-H3-23
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    36..88
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      496..505
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(535..738)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(564..584)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
       61 actgcagaat tcgaagcttg agctcgagat ccgctctttc tccgcgaatg cggcgagcga
      121 gctggatgtc tttgggcata atagtcactc gcttagcatg gatggcgcaa aggtttgtgt
      181 cctcaaagag ccctaccaag taggcctcac aagcctcctg cagcgccatc accgcagagc
      241 tctggaagcg aagatcggtc ttgaagtctt gggcgatttc tcgcaccagg cgctggaacg
      301 gcagcttccg aatcagcaac tcggtcgact tttggtagcg gcggatctcg cgcagagcca
      361 cagtgcccgg gcggtaacgg tgaggctttt tcacgccgcc ggtagccggc gcgctcttgc
      421 gagcagcctt ggtagccagc tgcttgcgtg gcgctttacc gccggtggat ttccgagctg
      481 tctgtttagt acgagccatg gtggcggcta gcggatctga cggttcacta aaccagctct
      541 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      601 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
      661 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
      721 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
      781 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
      841 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
      901 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
      961 a
//
