LOCUS       sequence-89357-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP3
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(104..110,111..128,129..134))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(159..283)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(320..364)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(377..397)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(452..473)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     801..820
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
       61 ttagctcctg aaaatctcga cggatcctaa ctcaaaatcc acacattata cgagccggaa
      121 gcataaagtg taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg
      181 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
      241 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgttgatatc tgcagaattc
      301 caccacactg gactagtccg ctgtccatgt gctggcgttc gaatttagca gcagcggttt
      361 ctttcccgcc cccgccgccc tgaaaataaa gattctcccc gcccccgccc ttgtacagct
      421 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
      481 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
      541 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
      601 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
      661 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
      721 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
      781 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
      841 cctggacgta gcc
//
