LOCUS       sequence-89359-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Leisinger AK, Janzen DH, Hallwachs W, Igloi GL
  TITLE     Amino acid discrimination by the nuclear encoded mitochondrial 
            arginyl-tRNA synthetase of the larva of a bruchid beetle (Caryedes 
            brasiliensis) from northwestern Costa Rica.
  JOURNAL   Insect Biochem Mol Biol. 2013 Dec;43(12):1172-80.
  PUBMED    24446543
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect 
            Biochem Mol Biol."; date: "2013-12"; volume: "43(12)"; pages: 
            "1172-80"
COMMENT     Sequence Label: pET-Cb2
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atcctcacat tgcttgtaag ggctgaagac
      121 ctagtatttc cataccattc ttcaatactt ccctggcagt gttaaaaagt aatagtcttt
      181 gggatgctac atctggactc atacctttga cttgtagttc atggagtgcc ttgttgatgt
      241 agttgcacaa gtgaaacagg tagttcacca atatatacgc ttctaactgc gcatttgcct
      301 tatataacac atcatgaaac tttgccagaa ctttcagcaa taccaacgct tcgggatcat
      361 cgaaaatctc tggattacat tctttggcag gtgttgctcc agaattcttt tccaggctat
      421 gtaagcgaca gtgtgtgtac tgaagcctta ttccagtgtc tccctgaacc tgaagtactg
      481 tatcccagtt gaactcgtaa tccttttgtc ttcgtctttt taactcatta ataattacac
      541 aggacacgcc caggatgtct gctacttcac catcattgat aggtaccttg gtagtaggtg
      601 actcaacttg tcgttttatc atgatatccc tgcattcatc cagaatatct ttaagaaaga
      661 tagatgttcc ttttcttgta ctcatacccc ttatcctacc aaacttgaca tgaactaatc
      721 tccctgccca aggtaagtcc attttatata gaatatcctt aagtgtataa agatgatcac
      781 tctggctgtt gtcaacaaca taatacatct tatcaaaact gtattttttg aatctatcta
      841 ttgctgcagc tatatcgcga gttagatata aggtagaacc atcactcttc agaactgaca
      901 cctttttttc atgtatagta gcaacttctt ttccatcatt acttttctgt ataatgttct
      961 tagttctcaa catttcaata acatttccaa tgttctttgc actatagtct gattcataac
     1021 tatagtcatc aaatgatat
//
