LOCUS       sequence-89360-e         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP3
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             72..116
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    153..277
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(302..307,308..325,326..332)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(452..471)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagggcg ggggcgggga gaatctttat tttcagggcg
       61 gcgggggcgg gaaagaaacc gctgctgcta aattcgaacg ccagcacatg gacagcggac
      121 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gctgaacgag
      181 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      241 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg
      301 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag
      361 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
      421 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
      481 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
      541 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
      601 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
      661 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
      721 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
      781 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
      841 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
      901 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggt
//
