LOCUS       sequence-89363-e        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP3
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(62..81)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(63..81)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          107..310
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(130..150)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat ggtaccaagc ttacctagcc agcttgggtc
       61 tccctatagt gagtcgtatt agtaccaagc taattcctca cgacacctga aatggaagaa
      121 aaaaactttg aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg
      181 caaattccaa ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact
      241 cagtgtgggg aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc
      301 aaaagctcga gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg
      361 aagcgacggc tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct
      421 ctcccgagcg ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc
      481 tttccgctca cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac
      541 acggcggcgc gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga
      601 ttctcggtgg ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg
      661 ccccgtcgcc gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat
      721 ttacaagact atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg
      781 ctagagactt atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc
      841 ggccccagcg cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc
      901 aaggcctcga actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact
      961 gcagccaggt ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc
     1021 cgcgggaacc acacacggca
//
