LOCUS       sequence-89364-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  split Venus attached to MCP and PCP respectively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Wu B, Chen J, Singer RH
  TITLE     Background free imaging of single mRNAs in live cells using split 
            fluorescent proteins.
  JOURNAL   Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615.
  PUBMED    24402470
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-01-9"; volume: "4:3615. doi"; pages: " 
            10.1038/srep03615"
COMMENT     Sequence Label: ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
       61 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
      121 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
      181 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
      241 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
      301 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
      361 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
      421 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
      481 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
      541 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
      601 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
      661 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctgg
//
