LOCUS       sequence-8937-ex        2540 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Oct;36(18):e114. doi: 10.1093/nar/gkn486. 
            Epub 2008 Aug 1.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008-10"; volume: "36(18):e114. doi"; pages: " 
            10.1093/nar/gkn486. Epub 2008 Aug 1"
COMMENT     Sequence Label: PL-452 N-CyPet
FEATURES             Location/Qualifiers
     source          1..2540
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..714
                     /codon_start=1
                     /product="cyan fluorescent protein optimized for FRET with 
                     YPet (Nguyen and Daugherty, 2005)"
                     /label=CyPet
                     /translation="MSKGEELFGGIVPILVELEGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSVMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKARHNITDGSVQLADHYQQNTPIGDGPVILPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     protein_bind    715..748
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        782..1281
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(817..840)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1214..1233
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        1293..1340
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1359..2162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1422..1441)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2032..2051
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2194..2211)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2200..2424
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2481..2514
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atgtctaaag gtgaagaatt attcggcggt atcgtcccaa ttttagttga attagagggt
       61 gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacgtacggt
      121 aaattgacct taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta
      181 gtcactactc tgacttgggg tgttcaatgt ttttctagat acccagatca tatgaaacaa
      241 catgactttt tcaagtctgt catgccagaa ggttatgttc aagaaagaac tatttttttc
      301 aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt
      361 aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa
      421 ttggaataca actatatctc tcacaatgtt tacatcaccg ctgacaaaca aaagaatggt
      481 atcaaagcta acttcaaagc cagacacaac attaccgatg gttctgttca attagctgac
      541 cattatcaac aaaatactcc aattggtgat ggtccagtca tcttgccaga caaccattac
      601 ttatccactc aatctgcctt atctaaagat ccaaacgaaa agagagacca catggtcttg
      661 ctcgaatttg ttactgctgc tggtattacc catggtatgg atgaattgta caaaataact
      721 tcgtataatg tatgctatac gaagttatgc tagcttggct gcaggtcgtc gaaattctac
      781 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
      841 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
      901 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
      961 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     1021 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     1081 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     1141 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     1201 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     1261 tcctcatctc cgggcctttc gacctgcagc ctgttgacaa ttaatcatcg gcatagtata
     1321 tcggcatagt ataatacgac aaggtgagga actaaaccat gggatcggcc attgaacaag
     1381 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     1441 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     1501 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     1561 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     1621 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     1681 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     1741 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     1801 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     1861 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg
     1921 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     1981 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     2041 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     2101 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     2161 gaggggatca attctctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     2221 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2281 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2341 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2401 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgacta
     2461 gagcttgcgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     2521 cctcgagggg atccactagt
//
