LOCUS       sequence-89380-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Velkov T, Chuang S, Prankerd R, Sakellaris H, Porter CJ, Scanlon MJ
  TITLE     An improved method for the purification of rat liver-type fatty acid
            binding protein from Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Nov . 44(1):23-31.
  PUBMED    15914028
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2005 Nov . 44(1):23-31."
COMMENT     Sequence Label: pTrc99A-Rat FABP1
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(470..492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    481..497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(505..511,512..528,529..534))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cgccaaacag ccaagcttgc atgcctgcag gtcgactcta gaggatcctg taaaagaata
       61 tgaaatacag ccttgtctaa attctcttgc tgactctctt gtagacgatg tcacccagtg
      121 tcatggtatt ggtgattgtg tctccattga attcagtcac ggactttatg cctttgaaag
      181 ttgtcaccat tttattgtca ccctccatct taaccactgc cttgaccttt tccccagtca
      241 tggtctccag ttcgcactcc tcccccaagg tgaactcatt gtggatcacc ttggacccat
      301 aggtgatggt gagtttgact ttcttccctt catgcacgat ttctgacacc cccttgatgt
      361 ccttcccttt ctggatgagg tcctcaggca gacccatcgc cttcatgaag ggctcaaagt
      421 tctcttggct ctgcacttgg tacttgccgg agaagttcat ggtacctttc ctgtgtgaaa
      481 ttgttatccg ctcacaattc cacacattat acgagccgga tgattaattg tcaacagctc
      541 atttcagaat atttgccaga accgttatga tgtcggcgca aaaaacatta tccagaacgg
      601 gagtgcgcct tgagcgacac gaattatgca gtgatttacg acctgcacag ccataccaca
      661 gcttccgatg gctgcctgac gccagaagca ttggtgcacc gtgcagtcga tgataagctg
      721 tcaaaccaga tcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
      781 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      841 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaaacagct
      901 gattgcccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgg
      961 cccagcaggc gaaaaatcct ggtttgatgg tggttaacgg cggga
//
