LOCUS       sequence-89382-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  TITLE     Jacks Lab Unpublished Plasmids
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GFP.Cre empty vector
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        49..548
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(84..107)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     481..500
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             552..572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtagg aattggccgc attctaccgg gtaggggagg
       61 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
      121 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
      181 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
      241 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
      301 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
      361 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
      421 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
      481 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
      541 gacccgggat gcccaagaag aagaggaagg tgtccaattt actgaccgta caccaaaatt
      601 tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg atggacatgt
      661 tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc gtttgccggt
      721 cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg
      781 ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact atccagcaac
      841 atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca agtgacagca
      901 atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg
      961 caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca ctcatggaaa
     1021 atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggat
//
