LOCUS       sequence-89383-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for PE OCT4 enhancer activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: pGL3-human OCT4 PE-SV40-Luc
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(76..272)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      90..225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      121 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg
      181 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      241 cgggactatg gttgctgact aattgagatg cagatcgcag atctcgagtc cacagacctc
      301 tggcactgtg ccttcagggg ccagtcaccc tctcagctcc tcaaatttat tgaatgtgtg
      361 tgtggcgcta tccctcaatg catcaacagc cataagcaca atggccagct gctcccttat
      421 gccttccccc gatccatcca gaatcctagg cattcccatc ccgatactgg ccaaatccag
      481 ccaccccgca gcctgggtgc ctggcaccat ctgcccagcc tgccaaattt caccccatct
      541 tcaagagtag actgccagac aaggcctccg tgctatatcc ccccaccccc catcccccca
      601 cccctccgtc ttccagaatc agactccaga ctctcctcat ctaacagact aaggggttgg
      661 cccctacttc cccttcaagg gaccagactt tggactgatt gggcctcagt ttcccaacct
      721 ttgctgaaac agagtgataa gacacccgct ttgggccccc tccactatgg aacctgcaca
      781 tcaggttcct tgctcccctc tcaaccaaaa ctcagacatc taataccacg gtaggcc
//
