LOCUS       sequence-89389-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used to mutate genes in Toxoplasma gondii using 
            the CRISPR/Cas9 system after having an appropriate protospacer 
            cloned into it..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Lourido lab Toxoplasma CRISPR Plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pU6-Universal
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(323..343)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(350..415)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
       61 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
      121 cctcggctgt ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt
      181 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
      241 tcacggccca gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcgg
      301 ctgctgggac tccgtggata ccgaccttcc gcttcttctt tggggccatc ttatcgtcat
      361 cgtctttgta atcaatatca tgatccttgt agtctccgtc gtggtcctta tagtccatgg
      421 tgtcgaaaaa gggaattcaa gaaaaaatgc caacgagtag ttttccgcaa gaacttcaca
      481 caaggtgatt gtgtaacacc gtttggtgga tgtcttctgc gcgggttgcg tcgtcaggga
      541 cggcgaacga gtgcttgttc tctacgagac tcgcaaagag aaggaaaggg cgaaattcaa
      601 gagagaatcc aacaaggagg actctctgct aatattcgcc gcctttaaca catctaaaag
      661 ttcacagaga acaaacaaaa tgtttctgac acatcccctt cgtgcgcgcg catctccacc
      721 ggtccaacca ggatcaccaa ccgcacccaa tgcaggcacc acccctgttc tgcgatgagc
      781 gcaagccgtc ctgagttacg ccttcggcat ctgactgccc accgcgagcc ggcctgctcc
      841 gcaacgtcat ccc
//
