LOCUS       sequence-89399-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains a single replication origin (ARS1) used for plasmid 
            replication assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Rizzardi LF, Dorn ES, Strahl BD, Cook JG
  TITLE     DNA replication origin function is promoted by H3K4 di-methylation 
            in Saccharomyces cerevisiae.
  JOURNAL   Genetics. 2012 Oct;192(2):371-84. doi: 10.1534/genetics.112.142349. 
            Epub 2012 Jul 30.
  PUBMED    22851644
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.112.142349"; journalName: "Genetics"; date: 
            "2012-10"; volume: "192"; issue: "2"; pages: "371-84"
COMMENT     Sequence Label: YCpLac111
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..78)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(70..92)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gccagcttgc atgcctgcag ggtcgactct agaggatccc cgggtaccga gctcgaattc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      241 tacgcatctg tgcggtattt cacaccgcat aatcgctggg ccattctcat gaagaatatc
      301 ttgaatttat tgtcatatta ctagttggtg tggaagtcct aatatcggtg atcaatatag
      361 tggttgacat gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat
      421 tttacaatca aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc
      481 ttataaaaaa gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt
      541 atctatgctg tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc
      601 cgggtaaata catataatac acaaatctgg cttaataaag tctataatat atctcataaa
      661 gaagtgctaa attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat
      721 atcgactttg taaaagttca cattagcata catatattac acgagccaga aatagtaact
      781 tttgcctaaa tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat gcttataatc
      841 aactttttta aaaatttaaa atactttttt attttttatt tttaaacata aatgaaataa
      901 tttatttatt g
//
