LOCUS       sequence-8940-ex        2501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Oct;36(18):e114. doi: 10.1093/nar/gkn486. 
            Epub 2008 Aug 1.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008-10"; volume: "36(18):e114. doi"; pages: " 
            10.1093/nar/gkn486. Epub 2008 Aug 1"
COMMENT     Sequence Label: PL-452 N-mDsRed
FEATURES             Location/Qualifiers
     source          1..2501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..675
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     399..418
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    676..709
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        743..1242
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(778..801)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1175..1194
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        1254..1301
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1320..2123
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1383..1402)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1993..2012
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2155..2172)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2161..2385
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2442..2475
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atggacaaca ccgaggacgt catcaaggag ttcatgcagt tcaaggtgcg catggagggc
       61 tccgtgaacg gccactactt cgagatcgag ggcgagggcg agggcaagcc ctacgagggc
      121 acccagaccg ccaagctgca ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      181 ctgtcccccc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      241 gactacatga agctgtcctt ccccgagggc ttcacctggg agcgctccat gaacttcgag
      301 gacggcggcg tggtggaggt gcagcaggac tcctccctgc aggacggcac cttcatctac
      361 aaggtgaagt tcaagggcgt gaacttcccc gccgacggcc ccgtaatgca gaagaagact
      421 gccggctggg agccctccac cgagaagctg tacccccagg acggcgtgct gaagggcgag
      481 atctcccacg ccctgaagct gaaggacggc ggccactaca cctgcgactt caagaccgtg
      541 tacaaggcca agaagcccgt gcagctgccc ggcaaccact acgtggactc caagctggac
      601 atcaccaacc acaacgagga ctacaccgtg gtggagcagt acgagcacgc cgaggcccgc
      661 cactccggct cccagataac ttcgtataat gtatgctata cgaagttatg ctagcttggc
      721 tgcaggtcgt cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
      781 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
      841 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
      901 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
      961 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     1021 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     1081 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     1141 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     1201 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cctgttgaca
     1261 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     1321 tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     1381 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     1441 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     1501 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     1561 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     1621 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     1681 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     1741 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     1801 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     1861 tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     1921 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     1981 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     2041 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     2101 gccttcttga cgagttcttc tgaggggatc aattctctag agctcgctga tcagcctcga
     2161 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2221 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2281 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2341 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     2401 gaaccagctg gggctcgact agagcttgcg gaacccttaa tataacttcg tataatgtat
     2461 gctatacgaa gttattaggt ccctcgaggg gatccactag t
//
