LOCUS       sequence-89402-e         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Cofilin-N-10
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      554..563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(605..626)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(866..885)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgtcgccac catggcctcc ggtgtggctg tctctgatgg
       61 tgtcatcaag gtgttcaacg acatgaaggt gcgtaagtct tcaacgccag aggaggtgaa
      121 gaagcgcaag aaggcggtgc tcttctgcct gagtgaggac aagaagaaca tcatcctgga
      181 ggagggcaag gagatcctgg tgggcgatgt gggccagact gtcgacgatc cctacgccac
      241 ctttgtcaag atgctgccag ataaggactg ccgctatgcc ctctatgatg caacctatga
      301 gaccaaggag agcaagaagg aggatctggt gtttatcttc tgggcccccg agtctgcgcc
      361 ccttaagagc aaaatgattt atgccagctc caaggacgcc atcaagaaga agctgacagg
      421 gatcaagcat gaattgcaag caaactgcta cgaggaggtc aaggaccgct gcaccctggc
      481 agagaagctg gggggcagtg ccgtcatctc cctggagggc aagcctttga gctccggagg
      541 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      601 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      661 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
      721 gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg
      781 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
      841 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
      901 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
      961 cgg
//
